| Objective:Nowadays,osteosarcoma is one of the most threatening malignancies,which seriously threatens human life and health.However,the efficacy of current standard therapies for osteosarcoma is not satisfactory enough,and it is accompanied by complications that seriously affect the quality of life of patients.Recently,Bismuth(Bi)-based nanoagents for synergistic photodynamic therapy(PDT)and photothermal therapy(PTT)are attracting attention and are highly desired for malignant tumor such as osteosarcoma diagnosis and treatment.But most of these materials are expensive or difficult to synthesize.Hence,producing these materials is still a challenge.Here,a facile solvothermal method is used to synthesize AgBiS2 and Tween-20(polyoxyethylene sorbitan monolaurate)is used for surface modification.In addition,the characteristics,biocompatibility,photothermal effect,photodynamic effect,CT imaging,antibacterial and tumor ablation capacity of NPs will be studied.Method:The AgBiS2 NPs were synthesized via a facile solvothermal method and Tween-20 was introduced for the surface modification.The characteristics of NPs were observed by high-resolution transmission electron microscope,and the crystal structure and purity were studied by an X-ray diffraction.Then,the biocompatibility of AgBiS2 NPs was tested by standard cell counting kit-8(CCK-8)method,hemolysis test and H&E staining.Besides,the optical absorption spectra were obtained by a spectrophotometer.The heating/cooling experiments were used to calculate the photothermal conversion rate of NPs.To verify photodynamic effects of NPs,the production of hydrogen peroxide(H2O2),singlet oxygen,and reactive oxygen species(ROS)in UMR-106(osteosarcoma cell line)cells were measured.The CT contrast(HU value)of NPs at was measured by CT scanning of mice,and CT scan of NPs in osteosarcoma to study its in vivo CT imaging capabilities.Cytotoxicity experiments and live/dead staining experiments after photothermal/photodynamic therapy for UMR-106 cells were used to study the ablation capacity of osteosarcoma in vitro.The photothermal/photodynamic therapy for osteosarcoma in nude mice was used to determine its ablation capacity in vivo.Co-culture of different concentrations of NPs with clinically common Staphylococcus aureus to calculate their antibacterial properties.Results:We have prepared fingerprint-like AgBiS2 NPs with an appropriate size of≈80nm.The NPs with good biocompatibility could effectively convert light into heat(with a high photothermal conversion efficiency of 36.51%)and significantly increase the generation of intracellular ROS under near infrared(NIR)laser irradiation.Remarkably,the combined PTT/PDT successfully inhibited the growth of a highly malignant osteosarcoma in vivo.In addition,AgBiS2 NPs exhibited an enhanced CT contrast ability for tumor imaging and outsdanding antibacterial abilities that the NPs could kill 95%Staphylococcus aureus at a concentration of 250μg/mL and could kill all Staphylococcus aureus when the concentration is higher than 500 μg/mL.Conclusion:Here,we have synthesized a multifunctional biocompatible AgBiS2 NPs with appropriate morphology and size via a facile solvothermal method.NPs show good photothermal and photodynamic effects,by which NPs can efficiently kill highly malignant osteosarcoma in vivo and in vitro.Besides,AgBiS2 NPs exhibited an enhanced CT contrast ability for tumor imaging and killed clinically derived Staphylococcus aureus(S.aureus)to prevent infection.In conclusion the ability of AgBiS2 NPs to be used in phototherapy and CT imaging and their antibacterial abilities indicate that they are promising candidates for malignant tumor theranostics. |
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