Effect Of Human Liver-Derived Stem Cells On Non-Alcoholic Fatty Liver

Objective: Analysis of the therapeutic effect of human liver-derived stem cells combined with normal diet on non-alcoholic fatty liver.Methods: 35 clean-grade male C57 BL / 6 mice were divided into 3groups by random number table method.The normal control group was fed with basal feed(group N),and the PBS group was fed with fatty liver with high fat diet.After aliquots of PBS(group M)and human liver-derived stem cell transplantation group were fed with high-fat diet for fatty liver modelling,HLSCs(group S)were injected twice a week.After the intervention,the body weight and liver index of the mice in each group were measured,and the contents of serum ALT,AST,TBIL,TG,TC,HDL-C,LDL-C,TG and TC levels in the liver,and liver histopathological changes were examined.Results: The differences in serum ALT,AST,TBIL,TG,TC and liver TG and TC levels among the three groups were statistically significant.Pairwise comparison between groups,the serum ALT,AST,TG,TC and liver TG,TC in M group mice were significantly higher than those in N group,the differences were statistically significant(all P values <0.05);the S group was small ALT and AST in mouse serum were significantly lower than those in N group(both P values were less than0.001),but TG and TC in serum and liver tissues were decreased,but thedifferences were not statistically significant(all P values were more than0.05);S The serum ALT,AST,and TG of the mice in the group were significantly lower than those in the M group(all P values <0.05),and the degree of liver steatosis in the mice in the S group was significantly reduced compared with the M group.Conclusion: Intraperitoneal liver-derived stem cell transplantation has a significant therapeutic effect on non-alcoholic fatty liver in mice.