| Objective The guinea pig model of vitiligo was intervened by308 nm excimer light irradiation to observe the changes of autophagy expression in the epidermal tissue of the lesion area.The purpose of the study is to explore the effect of 308 nm excimer light on the expression of autophagy in the lesion skin areas of guinea pig with vitiligo.Methods Twenty healthy common grade color guinea pigs(brown hair on the back)were selected,Twenty guinea pigs were randomly divided into a healthy group and an experimental group.There were seven guinea pigs in the healthy group and thirteen guinea pigs in the experimental group.Two groups of guinea pigs were selected to depilate about 2 cm× 2 cm area on their backs respectively.in the healthy group,no special treatment was given.In the experimental group,5% hydrogen peroxide solution was applied on the hair preparation area twice a day.After 68 days of continuous application,it was observed that the skin of the guinea pigs in the experimental group became lighter and whiter.Visually determine the success of the modeling,and then randomly select one guinea pig from the healthy group and the experimental group,cut the skin of the back experimental area for HE staining,After confirming that the modeling was successful,the remaining 12 guinea pigs in the experimental group were randomly divided into two groups of equal number,namely the model group and the 308 nm excimer light group.Guinea pigs in the 308 nm excimer light group were treated with 308 nm excimer light irradiation for 6 weeks,and the remaining groups were not treated.Then the pigmentation of the skin in the three experimental areas was evaluated by naked eyes,and the skin tissues were obtained for HE staining.At the same time,the S-P method was further used to detect the protein expression of autophagy related genes LC3 B and P62,and the RT-PCR technology was used to detect the m RNA expression of autophagy related genes LC3 B and P62.Results1.The S-P method test results:The average IOD value of LC3 B in the healthy group was(0.0182 ± 0.0052)and the average IOD value of LC3 B in the model group was(0.0297 ± 0.0054).Compared with the two groups,the LC3 B value of the model group increased,and the difference was statistically significant(P <0.05).After six weeks of intervention,the average IOD value of LC3 B in the 308 nm excimer light group was(0.0421± 0.0076),which was higher than that in the model group(P <0.05).The average IOD value of P62 in the healthy group was(0.0356± 0.0280),and that in the model group was(0.0297± 0.0054).In comparison,the P62 value of the model group was lower than that of the healthy group(P<0.05).After intervention for 6W in the excimer group at 308 nm,the average IOD value of P62(0.0139± 0.0061)was significantly lower than that in the model group(P<0.05).2.The RT-PCR method test results:The expression of LC3 B in the healthy group was(1.094± 0.1288),and the expression of LC3 B in the model group was(1.528± 0.1790).Compared with the two groups,the LC3 B value of the model group increased,the difference was statistically significant(P<0.05).After six weeks of intervention,the expression of LC3 B in the 308 nm excimer light group was measured(2.304±0.2089),which was higher than that in the model group(P<0.05).The expression of P62 in the healthy group was(1.013±0.0580),and that of the model group was(0.676±0.0510).In comparison,the P62 value of the model group was lower than that of the healthy group(P<0.05).After intervention for 6W in the excimer group at 308 nm,the expression of P62(0.379±0.0360)was significantly lower than that in the model group(P<0.05).Conclusion:After 308 nm excimer light intervention,the lesion area of the vit iligo guinea pig model recolored significantly and 308 nm excimerlight can significantly up-regulate the expression level of autophagy-related genes in the lesion tissue,thus promoting the successful recoloration of the vitiligo guinea pig model skin. |
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