Effects Of Brucein D On Migration And Invasion Of Human Triple Negative Breast Cancer MDA-MB-231 Cells And Its Mechanism

Objective: To investigate the effect of BD on the migration and invasion of human triple negative breast cancer MDA-MB-231 cells in vitro and its possible mechanism.Methods: Using BD of different concentrations(100,50,25,12.5,6.25,3.13,1.56,0.78 and0.39 μ M)as experimental group and cisplatin(DDP)of the same concentration as positive control group,the activity of MDA-MB-231 cells was detected by MTT method,and the concentration range of BD with little effect on cell viability was selected.In the selected concentration range of BD,with 0.1%DMSO as the control group,the effects of various concentrations of BD on the migration and invasion of MDA-MB-231 cells were detected by Wound? healing assay and Transwell assay,and the expressions of E-cadherin,vimentin,β-catenin,PI3 K,AKT and p-AKT in cells were detected by Westernblot assay.Results: MTT results showed that BD inhibited cell viability in a concentration-and dose-dependent manner,with IC50 values of 40.805,5.84 and 2.364 μ M at 24,48 and72 h,respectively.The results of Wound? healing showed that at 24 h,the width of cell wound treated with different concentrations of BD(1,2,4 μM)was 425.44 ±13.82μm,460.53 ±12.16 μm and 549.12 ±14.15 μm,respectively,which was significantly higher than that of the control group(318.13 ±11.68 μm).The results of Transwell assay showed that the number of invasive cells in the groups treated with different concentrations of BD(1,2,4 μM)was 148.33 ±5.53,110 ±9.21,82.33 ±5.55 respectively,which was significantly lower than that in the control group(192.33 ±6.08).The results of Westernblot assay showed that BD significantly up-regulated the expression of E-cadherin and down-regulated the expression of vimentin,β-catenin,PI3 K and p-AKT,but had no significant effect on the expression of AKT.Conclusion: BD can inhibit the migration and invasion of MDA-MB-231 cells of MDA-MB-231 cells in a concentration-dependent manner.This inhibitory effect is not related to the anti-viability of BD,but may be related to the inhibition of EMT and PI3K/AKT signaling pathways by BD.