Effect Of DiDang Tang On P-eIF2α CHOP And GRP78 Protein Expression In Rats With Intracerebral Hemorrhage Based On Endoplasmic Reticulum Stress Injury

Purpose:This experiment uses animal experiments to study the effects of DiDang Tang(DDT)on PERK pathway-related proteins and gene expression in endoplasmic reticulum stress injury after intracerebral hemorrhage,so as to explore the protective effect of DDT on neural injury after intracerebral hemorrhage,and provide scientific evidence for the clinical treatment of DDT in cerebral hemorrhage Theoretical basis.Method:Sixty healthy Wistar rats were used as research objects.Rats with cerebral hemorrhage were made by autologous blood injection into the caudate nucleus.The rats were randomly divided into 5 groups:sham operation group,model group,and DDT low,medium,and high measurement group,12 in each group.The scoring method was used to score the neurological function of several groups of rats;the brain water content in the penumbra of the cerebral hemorrhage was determined using the Billot formula.The western blot method was used to compare the dynamic expression of eIF2α,p-eIF2α,GRP78 and CHOP proteins in the penumbra of cerebral hemorrhage in each group.The RT-PCR method was used to compare the mRNA expression of GRP78 and CHOP.Result:1.Compared with the model group,the Longa five-point scoring method showed that the neurological scores of the low,medium and high measurement groups of DDT Decoction were significantly reduced,which was statistically significant( < 0.05).2.Compared with the model group,the Billot formula calculation method showed that the brain water content in the low,medium and high measurement groups of DDT Decoction was significantly reduced,which had statistical significance( < 0.05).3.Compared with the model group,western blot analysis showed that the expression of p-eIF2α,GRP78 and CHOP protein in DDT low,middle and high measurement groups was reduced,which was statistically significant( < 0.05).4.Compared with the model group,RT-PCR detection showed that the mRNA expression of GRP78 and CHOP in DDT low,medium and high measurement groups was reduced,which was statistically significant( <0.05).Conclusion: It has been experimentally proven that DDT can significantly improve neurological deficits in rats with cerebral hemorrhage,promote hematoma absorption after cerebral hemorrhage in rats,and reduce brain water content.It is proved from the protein level that its mechanism of action may be through down-regulation of p-eIF2α,GRP78 and CHOP It is proved at the gene level that its mechanism may be through down-regulating the expression of GRP78 and CHOP mRNA.In summary,DDT can inhibit the activation of apoptotic-related signaling pathways,thereby inhibiting excessive endoplasmic reticulum stress injury,protecting neurons in the penumbra zone of cerebral hemorrhage,thereby reducing neuronal apoptosis.