Study On The Regularity Of Calcium-containing Traditional Chinese Medicine And Its Effect On Antipyretic Convulsion In Bai-Hu-Tang

Objective:Systematic analysis of the regularity of calcium-containing traditional Chinese medicine.To study the effect of Bai-Hu-Tang（BHT）on rats with febrile seizures（FS）.To investigate the effects of different calcium-containing Chinese herbs in BHT on FS model rats.Methods:Chapter one Discussing the Law of Calcium-containing Traditional Chinese Medicine Based on Data MiningThe"Chinese Pharmacopoeia","Chinese Herbal Medicine","Chinese Traditional Medicine Dictionary"and"Anhui Provincial Traditional Chinese Medicine Preparation Standard"were used to count 29 kinds of Chinese medicines containing mainly calcium.The original database.Clementine 12.0 software was used for association analysis,SPSS 23.0 software was used for cluster analysis and principal component analysis to discuss the general medicinal properties and main components of calcium-containing traditional Chinese medicine.Chapter two Effect of BHT on Fever Convulsion Model RatsThe FS model of juvenile SD rats was induced by a hot water bath at a constant temperature of 45℃.The experiment rats was divided into 7 groups,the blank group,the model group,the magnesium sulfate（Mg SO4）group,the ibuprofen group,and the BHT high,medium and low dose groups.10,a total of 70.Weigh rats before and after the experiment;record rat convulsion latency and seizure duration;HE staining to observe hippocampal injury in rats;enzyme-linked immunosorbent assay（ELISA）to detect Glu,Asp,TNF-αand IL-1βcontent and serum TNF-α,and IL-1βcontent;colorimetric method to detect the content of Ca²⁺in serum and brain tissue;drying method was used to measure the water content of rat brain tissue,and the total body water content（TBW）of the rats was measured using an experimental animal body composition analyzer Extracellular Fluid Content（ECF）,Intracellular Fluid Content（ICF）,Fat Mass（FM）,Free Fat Mass（FFM）,and Body Mass Index（Body Mass Index）,BMI)and other bioimpedance indicators.Chapter three Effects of different calcium-containing Chinese herbs in BHT on rats with febrile seizuresThe FS model of juvenile SD rats was induced by a constant temperature 45℃hot water bath.The experiment rats was divided into blank group,model group,Mg SO4group,ibuprofen group,BHT group,BHT except gypsum group,gypsum group,BHT except gypsum plus Ca SO4·2H2O Group,BHT except gypsum plus stone cassia group,BHT except gypsum plus scutellaria group,10 in each group,a total of 100.Weigh rats before and after the experiment;record rat convulsion latency and seizure duration;HE staining to observe hippocampal damage in rats;enzyme-linked immunosorbent assay（ELISA）to detect brain tissue Glu,Asp,TNF-αand IL-1βcontent and serum TNF-αand IL-1βcontent;colorimetric method to detect the content of Ca²⁺in serum and brain tissue;meanwhile,total body water（TBW）,extracellular fluid（ECF）,intracellular Bioelectrical impedance indicators such as Intracellular Fluid（ICF）,Fat Mass（FM）,Free Fat Mass（FFM）,and Body Mass Index（BMI）;Western blot was used to detect the expression of calmodulin（CaM）and aquaporin 4（AQP4）in the brain tissue of FS model rats in different BHT containing calcium.Results:Chapter one Discussing the Law of Calcium-containing Traditional Chinese Medicine Based on Data MiningThe results of correlation analysis showed that the traditional Chinese medicine containing calcium was cold,tastes salty,and returned to the liver meridian,and its main effects were Pinggan Qianyang and Anshenzhenjing.Cluster analysis showed that the traditional Chinese medicine containing calcium was mainly divided into calcium carbonate group,calcium phosphate group,and In the calcium sulfate group,the three accounted for 96.5%of the calcium-containing traditional Chinese medicines;the main components of the calcium-containing traditional Chinese medicines were estimated to be Ca²⁺,CO₃^2-,and PO₄^3-ions.Chapter two Effect of BHT on Fever Convulsion Model RatsThe results of correlation analysis showed that the traditional Chinese medicine containing calcium was cold,tastes salty,and returned to the liver meridian,and its main effects were Pinggan Qianyang and Anshenzhenjing.Cluster analysis showed that the traditional Chinese medicine containing calcium was mainly divided into calcium carbonate group,calcium phosphate group,and In the calcium sulfate group,the three accounted for 96.5%of the calcium-containing traditional Chinese medicines;the main components of the calcium-containing traditional Chinese medicines were estimated to be Ca²⁺,CO₃^2-,and PO₄^3-ions.Chapter three Effects of different calcium-containing Chinese herbs in BHT on rats with febrile seizuresThe weight of rats in Mg SO4 group,ibuprofen group,BHT group,gypsum group and BHT except gypsum plus Ca SO4·2H2O group was significantly higher than that of model group（P<0.01,P<0.05）.Compared with the model group,the convulsion latency of the ibuprofen group,Mg SO4 group,BHT group and gypsum group was prolonged,and the duration of convulsion was gradually shortened（P<0.01,P<0.05）.Significant pathological changes in the neuron morphology of the hippocampal regions of the model group rats.The ibuprofen group,Mg SO4 group,BHT group,gypsum group,and BHT except gypsum and stone cassia groups can significantly improve neuronal damage in the model rats.Compared with the model group,the levels of Ca²⁺,Glu,Asp,TNF-αand IL-1βin the brain tissue of rats in ibuprofen group,Mg SO₄ group and BHT group were all reduced to varying degrees（P<0.01,P<0.05）.Compared with the model group,the serum Ca²⁺of rats in the ibuprofen group,BHT group,gypsum group and BHT except gypsum plus cassia significantly increased（P<0.01,P<0.05）;serum TNF-αand IL-1βThe contents were reduced to varying degrees（P<0.01,P<0.05）.Compared with the model group,the brain tissue moisture content,TBW,ICF and FFM of the ibuprofen group,Mg SO4 group and BHT group were significantly reduced（P<0.01,P<0.05）,and the ECF,FM and BMI were significantly increased（P<0.01,P<0.05）.Compared with the model group,CaM in the Mg SO4 group,ibuprofen group,BHT group,BHT except gypsum group,BHT except gypsum plus Ca SO4·2H2O group,BHT except gypsum plus stone Cassia group,and BHT except gypsum plus armour group（P<0.01,P<0.05）.Compared with the model group,the Mg SO4 group,ibuprofen group,BHT group,BHT gypsum group,gypsum group,BHT gypsum plus Ca SO4·2H2O group,BHT gypsum plus cassia group,BHT gypsum plus scallion group AQP4 was significantly reduced（P<0.01）.Conclusions:1.Calcium-containing traditional Chinese medicine is cold,tastes salty,and belongs to the liver meridian.Its main effects are Pinggan Qianyang and Anshen Zhenjing.It is mainly divided into calcium carbonate,calcium phosphate and calcium sulfate.The main ingredients are Ca²⁺,CO₃^2-and PO₄^3-ion;Potential therapeutic effect on febrile convulsions and hypertension in children.2.The medium dose group of BHT can relieve the symptoms of convulsion and cerebral edema in model rats by reducing the serum and brain inflammatory factors in FS model rats,increasing the serum Ca²⁺content,and reducing the intracellular Ca²⁺content in brain tissues,and preventing the repetition of FS attack.3.BHT can alleviate the symptoms of convulsion and cerebral edema in model rats by reducing serum and brain inflammatory factors in FS model rats,increasing serum Ca²⁺content,reducing intracellular Ca²⁺content,and reducing the expression of CaM and AQP4.Prevent recurrence of FS.Different calcium-containing Chinese medicines cannot be used as substitutes for each other.The specific mechanism of action needs to be further studied.