Study On The Difference Of Aging Degree In COPD Patients With Different Viscera Deficiency

Objective: By detecting the telomerase activity and telomere length of peripheral blood monocytes in patients with different viscera deficiency COPD,the difference of aging degree in patients with different viscera deficiency COPD was observed.Methods: 71 patients with chronic obstructive pulmonary disease were included in the stable period,and 44 patients in the healthy control group.The patients were classified according to the criteria for viscera deficiency and syndrome differentiation of traditional Chinese medicine.The information collection form,(CAT)COPD evaluation test scale and(mMRC)dyspnea scale were completed to detect the lung function of the patients.Peripheral venous blood of patients in each group was collected and PBMC was separated.Peripheral blood telomere length of each group was measured by flow-fish method and telomerase activity of each group was measured by trap-pcr-elisa method.Results: 1.Telomerase activity in peripheral blood of COPD patients was significantly higher than that of the healthy control group,and telomerase positive rate in peripheral blood of COPD patients was significantly higher than that of the healthy control group.There was no difference in telomere length between the healthy control group and the COPD patients(P>,0.05).2.There was no difference in telomerase activity in peripheral blood of different degrees of airflow restriction(P>,0.05);There was no difference in telomerase activity and telomere length in peripheral blood of COPD patients with different smoking conditions(P>,0.05).3.There were significant differences between the three groups of mMRC(dyspnea index),FEV1,FVC and CAT in COPD patients with different viscera deficiency(P<0.05).There was no difference in telomerase activity and telomere length in peripheral blood among the three groups of COPD patients with viscera deficiency(P>0.05),in which the mean value of telomerase activity(OD value)was the highest in the lung-kidney qi deficiency group and the lowest in the lung-qi deficiency group,suggesting that with the aggravation of the degree of viscera deficiency,the telomerase activity in peripheral blood had a trend of increasing gradually.4.In different age stratification,the telomerase activities of the healthy group and the COPD group were significantly different in the middle-aged group(P<0.05),but not in the elderly group(P>0.05).5.Partial correlation analysis after adjusting for age found that in COPD patients,telomerase activity was positively correlated with the number of acute exacerbations in the past year(r = 0.24,p = 0.04).Telomere length was positively correlated with FEVI(r = 0.59,p = 0.04)and FVC(r = 0.84,p = 0.00).Conclusion: 1.This study preliminarily found that telomerase activity in peripheral blood of COPD patients was significantly higher than that of healthy normal people,which was mainly concentrated in the middle-aged group and positively correlated with the number of acute exacerbations in recent one year.The positive rate of telomerase in peripheral blood of COPD patients was significantly higher than that of healthy control group.Also found that patients with COPD peripheral blood telomere length shortening and pulmonary function(FEV1 and FVC)was significantly positively related to drop degree and the peripheral blood of telomerase activity,shorten telomere length can be used as predict disease progression and COPD acute risk indicators,reflect the diversity of the pathogenesis of COPD,complex,and it is not just a single inflammatory mechanisms,may change with aging,immune pathways related to abnormal change.2.This study found that with the progressive aggravation of the deficiency of viscera in COPD patients,there may be a gradual increase in telomerase activity associated with aging,there may be a gradual increase in telomerase activity associated with aging,one second of forced expiratory lung volume and forced lung capacity gradually decrease,objective interpretation of COPD chronic disease pulmonary asthenia,lung disease extends to the spleen,kidney deficiency and gradually aggravates the disease,accelerating the theoretical basis of organic aging.