Study On The Virulence Of Aspergillus Lentulus Using The Galleria Mellonella Animal Model

Objective:Aspergillus lentulus(A.lentulus)is a new species of Aspergillus fumigatus(A.fumigatus)group.The pathogen is highly pathogenic and its virulence is unknown.This experiment preliminarily makes an animal model of G.mellonella and investigates whether the virulence of A.lentulus is different compared with other fungal species and the virulence of in different concentrations.Methods:The cultured A.lentulus and other groups of fungal were prepared into different concentrations of fungal suspensions and injected into the group of G.mellonella,and cultured in a 30°C incubator for 240 hours.During this period,the survival rates,melaninization scores,and activity score were observed and draw the curves,and then make statistical analysis on the data within 72 hours of the 1×10~6CFU.Results:Although A.lentulus was a sister fungus of A.fumigatus,compared with A.fumigatus,its colony and microscopic characteristics are unique.The survival rates,melaninization and activity scores curves of larvaes with different fungal have different downward trends.In general,the virulence of A.fumigatus and Candida albicans(C.albican)was stronger than the A.lentulus,and there was no significant difference in the the same species of A.lentulus.The survival indexs of infected larvae with A.lentulus suspensions at different concentrations are concentration-dependent and time-dependent.The higher the concentration,the longer the observation time,and the survival indexs would be lower.Conclusion:In conclusion,we have demonstrated that G.mellonella can be infected by strains as a model of fungal infection,and that this model reproduces the virulence phenotype observed in amoeba and mammalian infection models,and the cause of poor prognosis in patients with A.lentulus infection.There may be little correlation with virulence.The possible cause is the drug resistance of the strain,but the mechanism is still uncertain,so the research on the infection,immunity and drug resistance genes of the fungal will continue.