Study On Familial Aggregation Of Cerebral Thrombosis Susceptibility Gene Of P₂Y₁₂ And PF₄

Objective:To study on the differences in P₂Y₁₂2 and PF₄ gene expression during familial aggregation of cerebral thrombosis patients,and healthy people,and explore whether P₂Y₁₂and PF₄ gene are susceptibility gene of familial aggregation of cerebral thrombosis.Methods:We has studied out that the expression of P₂Y₁₂2 and PF₄ in familial aggregation of cerebral infarction patients have significant differences with normal,the prese-nt clinical collection including 25 familial aggregation of cerebral infarction,15 spor-adic patients with cerebral infarction and 15 normal people.we need their medical records and the venous blood,then extract RNA from human blood,reverse-transcription into cDNA,then using real-time fluorescence quantitative PCR technol ogy,illuminate the relative expre-ssion of target gene P₂Y₁₂2 and PF₄ of different groups of sample.At last,by statistical analysis,we can conclude whether the difference of target gene expression has statistical significance.Result:1.Expression of P₂Y₁₂2 gene in familial aggregation of cerebral thrombosis groups were significantly up-regulated than in sporadic cerebral thrombosis group and normal group,and the difference has statistical significance（P<0.05）.2.Expression of the PF₄ gene in familial aggregation of cerebral thrombosis group and sporadic cerebral thrombosis group and normal group has no statistical significance（P>0.05）.Conclusion:1.P₂Y₁₂2 gene may be related with familial aggregation of cerebral thrombosis,the absence or low expression of this gene can increase the susceptibility of cerebral thrombosis.2.PF₄ gene maybe not susceptible genes of familial aggregation of cerebral thromb-osis.