Effects Of Mifepristone And Misoprostol On The Ex-Pression Of TRIM22 In Trophoblast Cells

Research backgroundMifepristone(MIF)is the first dnug in the world to terminate pregnancyThe sequential use of misoprostol improves the success rate of medical abortion.It was widely used to terminate early and mid-term pregnancy.Induction of labor also has potential application value.The anchorage proliferation and differantiation of trophoblasts,the implantation of embryos and the formation of placenta were the key to the normal preg-nancy process.Mifepristone,as a powerful progesterone receptor(PR)and glucocorticoid receptor(GR)antagonist can effectively antagonize these two hormones,inhibit the anchoring proliferation and differentia-tion of trophoblasts,affect decidual and chorionic extracellular matrix remodeling,induce apoptosis at ma-temal-fetar interface,and then cause gaps,bleeding and embryo anchoring failure to fall off,In addition mife-pristone antagonizes the biological effect of progesterone on uterine contraction which leads to pregnancy termination.Although trophoblast cells belong to nomal tissue cells during embryonic development they are similar to tumor cells in biological behaviors such as proliferation,differentiation invasion and migration.Some scholars have described the similarity between the cytoplasmic trophoblast cells and the biological behavior of tumor cells from the aspects of cell proliferation and migration,invasicon signal transduction pathway,vascular invasion angiogenesis and apoptosis.TRIM protein was involved in the proliferation differentiation,apoptosis and immune response of various cells especially in the process oftumor development and devel-opmentTherefore the biological behavior of human trophoblast cells in early pregnancy is highly likely to be regulated by TRIM proteinPart One Effects of mifepristone and misoprostol on the expression of TRIM22 in chorionic villi of early pregnancyObjectiveTo detected the expression of TRIM22 in chorionic villi of embryo abortion group,artificial abortion group and drug abortion group.And further to explore the possible mechanism of mifepristone and miso prostol in drug abortion.MethodsUsing human pregnancy villus tissue samples as the research object.The chorionic villi of 40 cases of ear-ly pregnancy were divided into embryo abotion group(gioup A rF12),artificial abortion group(group B,n=14)and drug abortion group(group C,n=14).Immunohistochemistry was used to detect the expression site of TRIM22 protein and semi-quantitatively analyze its expression intensity.The expression levels of TRIM22 protein and mRNA detected by Westem blot and real-time quantitative PCR.Results TRIM22 protein was mainly expressed the nucleus of a cytotrophoblast The expression intensity of TRIM22 protein and mRNA in group A and C was significantly lower than that in group B(P<0.05),and there was no significant difference between group A and C(P>0.05).Conclusion TRIM22 was expressed in villus of early pregnancy,and it was highly expressed in villus of normal early pregnancy.The expression of TRIM22 decreased with the combination of mifepristone and misoprostol,and reached the same level as that of embryo sterilization grnup.These results suggest that mifepristone combined with misoprostol can reduce the level of TRIM22 in villus of early pregnancy,and may achieve the goal of apoptosis of embryo trophoblast cells by regulating the expressiono f TRIM22 in villus.Part Two Effects of mifepristone and misoprostol on the expression of TRIM22 in HTR-8 TrnphoblastsObjectiveTo detect the effects of mifepristone and misoprostol on the proliferation migration and TRIM22 expres-sion of HTR-8 trophoblasts.To futher explore whether there was significant interaction between mifepris-tone and misoprostol in the regulation of TRIM22 expression.MethodsExtravillous trophoblast,constructed by HTR-8cells was studied.The experiment was randomly divided into four groups according to two-factor factorial design.the blank control group(group D),the misoprostol group(group E),the mifepristone group(group F),and the mifepristone plus misoprostol group(group G).The proliferation ability of each group was detected by cck-8 method.The migration ability of each group was detected by cell scratch test.The expression levels of TRIM22 protein and mRNA were detected by Westem blotting and real-time quantitative PCR.ResultsCompared with those without mifepristone(groupDandE),the proliferation and migration ability of trophoblasts significantly decreased,and the expression of TRIM22 protein and mRNA was signifi-cantly decreased in the mifepristone group(groupFandG)(P<0.001).There was no statistically significant difference between the group using misoprostol(groupEandG)and the group without miso-prostol(groupDandF)(P>0.05);there was no significant interaction between the two drugs(P>0.05).CondusionMifepristone inhibited the proliferation and migration of trophoblast cells and down-regulated the expres-sion of TRIM22 in trophoblast cells.Mispostol had no statistically significant efect on its effect Mifepris-tone may down-regulate the expression of TRIM22 and participate in the prolifeation and migration of trophoblast to achieve the purpose of apoptosis.