Testing Of Behavioral And Cognition Development In The Rat After Prenatal Exposure To 1800 And 2400 MHz Radiofrequency Fields

ObjectiveTo explore the effects of Behavioral and Cognition Development in the Rat after Prenatal Exposure to 1800 and 2400 MHz Radiofrequency Fields.Method18 Wistar female rats and 9 male rats at age of 6 weeks old were randomly divided into six groups(3 rats in each)after two week for environmental adaptation and successful mating.The experimental groups were:1800MHz exposure group(power density of 1.0mW/cm2);1800MHz+WiFi exposure group(power density:1.0mW/cm2);WiFi exposure group(power density 0.1mw/cm2).The exposure time was from 0 pregnant day to 20 pregnant days,every night 20:00 to 8:00 in the morning the next day.Simultaneously,the virtual exposures were set as controls for the there experimental groups.Rats in each group were all free to drink and eat during exposure,and the environmental background conditions(including temperature,humidity,background noise,aeration,lighting)were stable.After the completion of exposure,the indicators of physiological development and behavioral development were observed and measured in offspring rats.When the offspring were three weeks old,Y maze was measured,Y maze and open field were measured at seven weeks old,and the hippocampal tissue was measured at eight and nine weeks old by the skinning box.At ten weeks old,the mRNA and protein expressions of NMDARs were measured by rt-per and western-blot in rat hippocampus.Results1.Development indicators:physiological development indicators:the body weight of the 1800MHz exposure group and the 1800MHz+WiFi exposure group was different from that of the control group(p<0.05),and the body weight of the two groups showed a downward trend,and the open eye time of the WiFi exposure group was different from that of the control group(p<0.05).There was no statistical difference in other physiological development indexes.2.Behavioral indicators:(1)Y maze experiment:1)the total distance of the 1800MHz+WIFi exposure group is shortened,and the total rest time is increased;The number of wrong arm entry decreases and the rest time increases.The duration of the error arm in the WiFi exposure group was shortened and the average speed was increased.The rest time of the food arm increased(p<0.05).2)7-week-old offspring Y maze experiment:the total distance,average speed and total rest time of the WiFi exposure group were shortened;The distance of the starting arm is shortened and the duration is reduced.Error arm entry reduced;The duration and rest time of the food arm increased,and the average velocity decreased(p<0.05).(2)Open-field experiment:in the 1800MHz+WiFi exposure group,the distance,duration,access times and rest time of the central area were all higher than other groups;There was no difference between other groups.The distance,duration and entry times of the offspring in the surrounding area in the 1800MHz+WiFi exposure group were lower than those in other groups(p<0.05).The incubation period was higher than other groups(p<0.05).There was no difference between other groups.(3)Skinner box experiment:there was no significant difference in the scores of each group under the same program,and there was no significant difference in the scores of light and dark resolution of each group under the same program.3.MRNA relative expression levels of seven subunits of NMDARs in the hippocampal tissues of 10-week-old rats:the mRNA relative expression levels of NR2A and NR2B in the 1800MHz+WiFi exposure group were down-regulated(p<0.05).MRNA relative expressions of NR2D,NR3A and NR3B were up-regulated(p<0.05).MRNA relative expressions of NR2A and NR2B in the WiFi exposure group were down-regulated(p<0.05).MRNA relative expressions of NR1,NR2C,NR2D,NR3A and NR3B were up-regulated(p<0.05).Expression levels of NMDAR2A and NMDAR2B in hippocampal tissues of 10-week-old young rats:the expression level of NMDAR2A protein was up-regulated in the hippocampus of 1800MHz exposure group(p<0.05).The protein expression of NMDAR2B was down-regulated in hippocampus of WiFi exposure group(p<0.05).Conclusion1.There was an effect on body weight and eyes opening time of offspring rats exposed to 1800MHz and WiFi radio-frequency field;while the impact on forelimb hanging test needed to be further verification.2.There was an effect on spatial learning ability of 3-week-old offspring rats exposed to 1800MHz and WiFi radio-frequency field;while the effect on spatial learning and memory of 7-week-old offspring rats needed to be further validated.3.rt-PCR and Western Blot test indicated that the expression of NMDARs in offspring rats hippocampus presented difference in experiment groups(The 1800MHz+WiFi expose group and WiFi expose group)compared to control groups.4.The effects of WiFi on offspring rats learning and memory ability remained unclear.