| Objective The research on the chemical constituents of Gynostemma pentaphyllum medicinal materials;the quantitative analysis method of common components was determined through the study of medicinal materials,standard decoctions and formula granules;the fingerprint method and TLC of Gynostemma pentaphyllum,standard decoction and formula granules were established respectively.Identify methods and establish relevant quality standards.Methods I.Field investigations were conducted on the distribution of Gynostemma resources and samples were collected.2.The chemical constituents of Gynostemma pentaphyllum from different habitats were studied.The chemical constituents of Gynostemma pentaphyllum were studied and confirmed by HPLC and HPLC-MS.3.HPLC method was used to establish the method for determination of Gynostemma penttrphyllum and fingerprint method,and the method was verified.The similarity of 34 batches of herbs was calculated by the similarity evaluation software.4.The method for determining the content of Gynostemma pentaphyllum and the method of fingerprinting were established,and the methodological verification was carried out.The similarity of’the standard decoction was calculated by the similarity evaluation software.The transfer rate of the standard decoction was calculated according to the content of the index components in Gynostemma pentaphyllum.5.Investigate the different preparation processes of Gynostemma formula particles and determine the best extraction process.6.The method for determining the content of Gynostemma pentaphyllum granules and the method for measuring fingerprints were established,and the methodological verification was carried out to calculate the similarity and transfer rate.7.The TLC methods was established for medicinal materials,standard decoctions and formula granules and Gynostemma pentaphyllum medicinal materials by TLC,and methodological investigation was carried out.8.Establish quality standardsResults 1.The source and name of Gynostemma pentaphyllum were examined,and the Gynostemma plant was simply summarized.34 batches of Gynostemma pentaphyllum,15 batches of Gynostemma pentaphyllum standard lyophilized powder and 18 batches of Gynostemma formula granules were collected to determine the production process of the formula granules.2.Through the study of the chemical constituents of Gynostemma pentaphyllum,the origin of Gynostemma pentaphyllum standard decoction and formula granules was determined.Six chemical components were identified by HPLC and HPLC-MS,and two of the compounds,gypenoside XLVI(C48H82O19)and ginsenoside Rb3(C53H90O22)were confirmed.3.For the first time,a method for simultaneous determination of gypenoside XLVI and ginsenoside Rb3 was established.The extraction method of the test solution was determined by comparing different solvents and different extraction methods.The methodological investigation met the requirements and was determined.The average recovery of gypenoside XLVI was 93%,the RSD%was 0.61%,the average recovery of ginsenoside Rb3 was 100%,and the RSD%was 2.32%.There are 7 characteristic peaks in the fingerprint of Gynostemma pentaphyllum,and 7 similarities are above 0.9.In comparison,it was found that the Gynostemma harvested in July of the 15 batches was lower than that of Gynostemma in October and November,indicating that the saponin content was higher in October and November of each year.4.For the first time,the content determination and fingerprinting method of Gynostemma pentaphyllum standard decoction were established.The average recoveries of gypenoside XLVI and ginsenoside Rb3 were 92%and 102%,respectively,and the RSD%were 1.43%and 0.79%,respectively.The average transfer rate of gypenoside XLV1 in the standard decoction was 76.5%,and the average transfer rate of ginsenoside Rb3 was 60.5%.There are 7 characteristic peaks in the established fingerprint of the decoction,and 9 similarities are above 0.9,which corresponds to the results of the determination of the medicinal materials.5.The process parameters such as extraction,concentration,drying and excipients were investigated,and the optimal preparation process of Gynoslemma formula granules was determined.The method for determination and fingerprinting of Gynostemma granules was established for the first time.The methodological results were good.The average transfer rate of gypenoside XLVI was 54.1%,and the average transfer rate of ginsenoside Rb3 was 72.3%.The nine characteristic peaks in the formulated particle fingerprint correspond to the medicinal material and the standard decoction.6.For the first time,the TLC methods for Gynoslemma penlaphyllum standard decoction and formula granules was established.The solvent,developer ratio,unfolding temperature and thin layer of the developing agent were investigated to determine the better conditions for the development of Gynostemma pentaphyllum.The decoction and formula granules and the can show the same color spots at the corresponding positions.Conclusion The origin of Gynostemma penlaphyllum were determined,and the origin of Gynostemma penlaphyllum standard decoction and formula granules was fixed in Wuyi,Zhejiang.The confirmed two components,gypenoside XLVI and ginsenoside Rb3,provide the basis for the study of saponin components of Gynoslemma pentaphyllum.The determination of the content of Gynostemma penlaphyllum,standard decoction and formula granules,fingerprinting and thin layer identification method provides the research basis for the quality control of Gynostemma pentaphyllum.The fingerprints of Gynostemma pentaphyllum,standard decoction and formula granules can be used for the research and authenticity identification of Gynos6emma penlaphyllum.From the source,the medicinal materials,standard decoction and formula granules are controlled in multiple steps,and the medicinal materials and standards are respectively formulated.The quality standards of the decoction and formula granules provide a reliable method for the quality control of Gynostemma pentaphyllum,which is beneficial to the authenticity identification of the finished product in the case of losing the appearance of the medicinal material. |
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