The Activity Of Vitamin E Regulating Human Protein Phosphatase-2A And The Action Of MiR-572

Background and purpose:Vitamin E may be closely related to cardiovascular diseases(CVD)such as hypertension and coronary heart disease.Vitamin E supplements reduce the incidence of CVD.Vitamin E activates protein phosphorylase-2A and dephosphorylates protein kinase C.MicroRNAs(miRNAs)are considered as potential targets for the treatment of various cardiovascular diseases,including atherosclerosis,myocardial infarction and hypertrophy.In this study,3 patients with atherosclerosis and 3 healthy volunteers as the CK were included for data measurement and analysis,to guide patients with CVD not to overdose on vitamin E and rational use of vitamin E in association with protein phosphatase-2A,and the effect of miR-572 was also analyzed.It is expected that it provides an important basis for the targeted treatment of CVD disease.Data in this study revealed miRNA and related target genes in patients with CVD,however,more patients need large-scale data collection and further investigation,molecular detection may contribute to the prognosis and treatment of cardiovascular diseases.The research conclusions are as follows:1.The content of vitamin E in serum samples of the experimental group and the control group was determined by HPLC,the quantitative analysis was carried out by internal standard method.And it turns out,the content of vitamin E in serum samples of patients was higher than that of normal volunteers.statistical analysis *P=0.045,statistically significant difference.Given the antioxidant properties of vitamin E,patients should take adequate supplements.2.Vitamin E activates protein phosphatase-2A,protein phosphatase-2a inactivates protein kinase C,it inhibits cell growth.Protein phosphatase-2A plays an important role in cell cycle regulation and signal transduction,thus controlling the activity of DNA replication and transcription.We speculate that VE regulates protein phosphatase-2a,thus indirectly affects the treatment of cardiovascular diseases.Human protein phosphatase(PP2A)ELISA kit was used to determine the content of human protein phosphatase-2A,and it turns out,the PP2 A enzyme content of the patients was higher than that of the volunteers,the difference was statistically significant(p=0.015).3.Protein phosphatase-2A was up-regulated in patients with CVD,mir-572 regulates PP2 A mRNA(encoding genes ENSG00000113575.9_,ENSG00000104695).MiR-572 sequence was obtained by sequencing.The expression level of miR-572 was detected by reverse transcription-quantitative polymerase chain reaction(RT-QPCR),The relationship between miR-572 and PP2 A was also analyzed.And it turns out,CD patients were 0.34 compared with CK fold change,the results of rt-qpcr were consistent with the sequencing results.mir-572 may regulate PP2 AmRNA,It plays a key role in the pathogenesis of CVD,we need further research to verify that,facilitate aggressive treatment of CVD.