Effects Of Adipose Tissue Specific Inhibition Of VEGFB On Energy Metabolism

Obesity,a chronic disease and an epidemic disease whose prevalence is increasing at an alarming rate,has been attracting global attention in recent years.Obesity is mainly caused by excessive accumulation of fat.Adipose tissues are mainly divided into two types,white adipose tissues are responsible for storing energy,and brown adipose tissues produce heat,and obesity is an excessive expansion of white adipose tissue.Vascular endothelial growth factor B(VEGFB)has been found to be closely related to energy metabolism.Many studies have shown that knockout of VEGFB leads to obesity and metabolic slowdown in the organism,but the relations between tissue-specific inhibition of VEGFB and metabolism is still not well understood.Studying the role of adipose tissue-specific inhibition of VEGFB in lipid metabolism is the focus of this experiment.The latest Crispr-dcas9 system was used in this paper,the fragment AP2-dcas9 was microinjected into mouse zygotes to get the adipose-specific VEGFB inhibition mice to study the role of VEGFB in fat tissues.First of all,we found the expression of Leptin and Resistin,which is related to white adipose tissue development,will increase when VEGFB is inhibited in adipose tissue.But the specific genes of adipose tissue,such as UCP1,BMP7,PRDM16,etc.,their expression levels will decrease.At the same time,the weight of transgenic mice was significantly higher than that of wild-type mice when food intake was almost the same.The mice were dissected,the gonads white adipose tissue was taken out and the weight was measured.It was found that the adipose tissue of the transgenic mice was significantly heavier than the wild type,and the body fat rate also showed the same trend.HE staining of adipose tissue revealed that the white adipose tissue and brown adipose tissue of the transgenic mice appeared whiter than WT mice,and the volume of the cells was larger,and the number of cells in the same area was significantly reduced.Adipose-specific inhibition VEGFB leads to control the development of adipose tissue through the changing expression of related transcription factors.Secondly,in terms of energy metabolism,this study found that fatty acid transporter 3(FATP3)is significantly elevated in WAT,but decreased in BAT when VEGFB was specifically inhibited in adipose tissues,resulting in fat accumulates in WAT and metabolic dysfunction in BAT.The body temperature was measured and found that the body temperature of the transgenic mice was slightly lower than that of the wild type.Determination of triglycerides and cholesterol revealed that triglycerides in transgenic mice were slightly elevated,while cholesterol had no difference in both groups.Also,glucose tolerance and insulin sensitivity were measured and found to be indistinguishable.Therefore,our study found that adipose tissue VEGFB repression can regulate the development of adipose tissue and have a certain effect on energy metabolism,but it does not induce a pathological effect.It is proved that adipose tissue-specific VEGFB inhibition in lipid development and metabolism is not completely consistent with the effects of systemic VEGFB knockout,which is of great significance in the treatment of obesity and its associated diseases,which also provides an important reference value for the study of targeted drugs under minimizing systemic whole body influence.