Micro-/Nanostrcture Regulation Of Zein Surfaces Using Electrospinning Technique And Its Effect On Cells

Thrombosis and intimal hyperplasia is the main reason that artificial blood vessels can not maintain long-term patency in vivo.Studies have shown that a complete endothelial cell monolayer on the lumen surface of artificial blood vessels can effectively reduce thrombosis and intimal hyperplasia.Therefore,one of the key points in the study of artificial blood vessels that we should focus on is how to enhance the adhesion of endothelial cells on the lumen surface.In this study,we used electrospinning technique to construct zein films composed of different micro-/nanostructures,and characterized the microscopic morphology of these films,(including of fiber microscopic morphology,diameters,orientations and anti-swelling modification effects.The hydrophilicity,surface roughness and protein adsorption were alsomeasured.Then the morphologies of different types of cells on these films were studied.The shear stress of about 15 dynes/cm~2 was provided by a self-made parallel plate flow chamber to simulate the erosion of blood on the vessel wall,and the adhesion capacities of different cells on zein films with different micro-/nanostructures were studied.The results showed that the zein electrospun fiber films could remarkably improve the retention of fibroblasts(L929);however,they did not improve the retention of endothelial cells(EAhy926).There is no significant correlation between the retention of EAhy926 cells on the fiber films and the diameter of fibers.