Effect Of Adhesion Protein PTPRM On Zn²⁺-mediated Neuronal Cell Growth And Synaptic Activity

The nervous system is one of the most important and complex system of organisms.The various neurological diseases caused by abnormalities of the nervous system seriously affect human health and daily life.At present,neurological diseases have caused widespread concern,and the conquest of such diseases has become the direction of researchers’ efforts,and exploring the pathogenesis of various neurological diseases is also an urgent problem to be solved.The most important role of the nervous system is to regulate the body’s various physiological activities by transmitting various types of information from the body.This process is extremely complicated.In the central nervous system,cell signals are mainly transmitted chemically,that is,vesicles envelop neurotransmitters,transport neurotransmitters to the presynaptic membrane,and release neurotransmitters through membrane fusion to bind to related receptors to achieve signal transmission.Zinc is one of the essential trace elements in organisms.The lack of zinc can have a great impact on organisms and cause a variety of diseases.Excessive zinc will lead to zinc poisoning and cause some damage to the body.We can find that zinc is closely related to the body’s functional metabolism and the occurrence of some neurological diseases.So,what are the specific factors that cause zinc to trigger neurological diseases in the body? This study focuses on this core issue.In order to explore the specific factors affecting the influence of zinc ions on nerve cells and the effect of this factor on the synaptic activity of nerve cells,this study uses gene chip technology to add appropriate amount of zinc ions during cell growth.After the cell growth is completed,the total RNA is extracted.After screening out a variety of differential genes,we selected the gene PTPRM associated with cell adhesion for further study.Then,PTPRM was overexpressed by nerve cells with zinc ion and zincfree ions.Laser confocal imaging and patch clamp technique were used to further explore whether PTPRM protein is associated with nerve cell growth and vesicle release.In this study,we explored the effects of PTPRM protein on nerve cell growth and signal transmission through a series of experiments.We found that the long-term effect of zinc ions caused the decrease of PTPRM protein content in nerve cells and the synaptic density.When PTPRM was replenished,the synaptic density of cells almost returned to normal level.This result indicates that the PTPRM protein is closely related to the growth of the cells to some extent.In the patch clamp experiment,it was found that the frequency of mEPSC and the amplitude of eEPSC in zinc-treated neurons were lower than those in normal neurons,and the recovery of PTPRM returned to normal levels,Therefore,we found that PTPRM protein was involved in the growth and synaptic activity of Zn²⁺ mediated neurons.