Studies On Chemical Constituents And Bioactivity Of Achyrocline Satureioides

Achyrocline satureioides(Lam.)DC.(Compositae),On the basis of previous studies,a systematic research on chemical constituents in Achyrocline satureioides have been given in this research by using silica gel column chromatography,ODS column chromatography,Sephadex LH-20 column chromatography and preparative HPLC.48 compounds were obtained from the ethyl acetate fraction.41 compounds has been identified by spectroscopy and physicochemical methods,as 12 compounds were first separated from Achyrocline:2-(1-oxopentyl)-benzoic acid ethyl ester,senkyunolide I,α-amyrin,pomolic acid,(4S,5R)-5-(4’-methyl-3’-pentenyl)-4-hydroxy-5-methyldihydrofuran-2-one,clovandiol,caryolane-1,9β-diol,(+)-(3R)-3-hydroxyl-4,4-dimethyl-4-butyrolactone,quercetin 4’-O-β-D-glucoside,hostaflavanone A,neosakuranin,3-O-methoxy quercetin-3’-O-beta-D-glucopyranoside;29 known compounds:5-hydroxy-3,7-dimethoxyflavone,diamyl phthalate,β-sitosterol,(S)-3-[{5,7-dihydroxy-2,2-dimeth-yl-8-(2-methyl butanoyl)-2H-chromen-6-yl}methyl]-6-ethyl-4-hydroxy-5-methyl-2H-pyran-2-one,β-sitostenone,ergosta-4,6,8(14),22-tetraen-3-one,3,5-dihydroxy-6,7,8-trimethoxyflavone,lepidissipyrone,7,4’-dihydroxy-5-metho xyflavanone,(24R)-24-ethylcholest-4-en-3,6-dione,1-(2-methylbutanone)-3-prenyl-ph loroglucinol,5,7-Dihydroxy-3,6-dimethoxyflavone,ursoli cacid,galangin-3-methyl ether,quercetin,anthemisol,pinocembrin,(2R,3R)-taxifolin,quercetin 3-methyl ether,protocatechuic acid,Helichrysetin,palmitic acid,methyl linolenate,galangin,genkwanin,4’-hydroxydehydrokawain,3,3’-dimethoxy quercetin,isorhamnetin,quercetin-7-O-β-D-glucoside.Through network pharmacology and tumor cell activity screening experiments,the active ingredients of Achyrocline satureioides including anti-inflammatory,anti-oxidation,anti-tumor and other activities were preliminarily determined.