| Objective Tendon injury is a clinically common type of injury..Since the tendon cells are surrounded by longitudinally arranged bundles of collagen fibers,the tendons have a strong tensile strength.However,tendon tissue has a small proportion of blood vessels,so it is not conducive to tissue repair and regeneration after injury.The fixation of the tendon during the healing process,although maximally reducing the chance of re-rupture of the tendon,may cause complications such as joint stiffness,which is not conducive to later functional exercise.The previous study of this group proved that the functional biomechanical properties of the functional position were better than those of the traditional extension position by measuring the functional external fixation and the related biomechanical indexes after the fixation.However,the intensity of the force at the end of the tendon and the change with time are still unclear,and no relevant reports have been reported at home and abroad.In recent years,in vitro and in vivo experiments of tendon are hot spots in the tendon field.The difference between the two is that in vitro experiments require the support of nerve,muscle and in vivo biological environment.In this experiment,the biomechanics of rabbit tendon was tested by Salmons ring-type sensor.The early evaluation of tendon tension changes provided experimental basis for tendon injury assessment.This study will further investigate the feasibility of functional fixation by measuring the changes in the early tension of the tendon at the end of the body tendon and the changes in the expression of related growth factors in the healing process of the Achilles tendon.Methods 100 adult male New Zealand white rabbits weighing 2-2.5 kg were divided into 4 groups according to a completely random method,which were labeled as group A,group B,group C and group D,respectively,30 in groups A and B,20 in groups C and D.It is proposed to perform "Achilles tendon acute fracture modeling + Achilles tendon rupture repair + Salmons sensor implantation surgery" on the right lower limb of 30 rabbits in group A.The postoperative functional position fixes the ankle joint of rabbit.It is proposed to perform "Achilles tendon acute fracture modeling + Achilles tendon rupture repair + Salmons sensor implantation surgery" on the right lower limb of 30 rabbits in group B.The ankle joint of rabbit is fixed in the extension position.The right lower extremities of40 rabbits in group C and group D were enrolled in the “Achilles tendon sensor implantation procedure”,and the ankle joints of the rabbits were fixed with functional and extension positions.The 5 selected rabbits in the 5th,30 th,2h,8h,24 h,48h,and 2 weeks of the sensor were randomly selected from the A,B,C,and D groups to measure the tension of the Achilles tendon(or Achilles tendon).At the peak of the 7th,14 th,21st,and 28 th day after surgery,5 rabbits in the A and B groups were randomly selected to detect the expression of related growth factor genes during the healing process of Achilles tendon,and to explore the early process of tendon healing after acute hemorrhoids in rabbits.Biomechanicalcharacteristics of injured tendon after a certain stress stimulation.Results 1.At 5 min,30 min,2 h,and 8 h after operation,the peak tension of the four groups of rabbit Achilles tendon was statistically significant(P<0.05).The peak tension of group A was the highest,the second group was the second,and the worst was group D.There was no significant difference in the peak tension between the four groups at 24 h,48h and 2 weeks(P>0.05).2.At 7 and 14 days after operation,the expression levels of type I collagen,type III collagen and IGF-1 in group A were higher than those in group B(P<0.05);group A and group B were 21 and 28 days after operation.The type I collagen gradually increased and replaced type III collagen.The expression levels of type III collagen and IGF-1 in group A decreased to some extent,but still higher than group B(P<0.05).Conclusion 1.The early functional fixation of rabbit Achilles tendon after acute fracture repair is more conducive to the improvement of biomechanical properties.It is proved that postoperative Achilles tendon is feasible under functional fixation,which provides more theoretical basis for functional external fixation.The expression level of related growth factor genes in group A was higher than that in group B at each time point,indicating that early fixation by functional position is a favorable factor for promoting Achilles tendon healing. |
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