| Objective To investigate the characteristics of tongue image,characteristics of colonoscopy and apoptosis of exfoliated cells in colorectal cancer patients with damp-heat syndrome,and to detect cell maturation value(MI,MV),positive for EGFR,casepase-3 and c-Jun protein.Expression rate.It is expected to find the correlation between the characteristics of tongue color of Chinese medicine,the characteristics of colonoscopy and the apoptosis of exfoliated cells of colorectal cancer.Provide a basis for early diagnosis of colorectal cancer,and broaden the research ideas of objective diagnosis of tongue diagnosis in traditional Chinese medicine.Methods In the future,patients who underwent colonoscopy in our hospital and who met the Chinese medicine diagnosis of this study were diagnosed as damp-heat accumulation syndrome as a case control group.Among them,patients with clear diagnosis of colorectal cancer were enrolled in the colorectal cancer group,a total of 60 cases;patients with a definite diagnosis of intestinal polyps were included in the polyp group,a total of 20 cases;patients with definitive diagnosis of ulcerative colitis were included in the ulcerative The colitis group(hereinafter referred to as the stagnation group),a total of 20 cases.A total of 20 patients were enrolled in the normal control group with normal physical examination and no intestinal lesions.After the colonoscopy results were subgrouped,the characteristics of the tongue and colonoscopy of the above observation objects were observed and analyzed,and the tongue coating was scraped and preserved.The maturity(MI,MV)of the exfoliated cells of the tongue coating was detected by Pap staining.The positive expression rate of EGFR,casepase-3 and c-Jun in exfoliated cells of tongue coating was detected by immunohistochemistry.Results 1.Tongue feature:The colorectal cancer group was characterized by purple tongue and thick yellow greasy moss;the polyp group was characterized by dark red tongue and thin yellow greasy moss;the stagnation group was characterized by red tongue and thin yellow greasy moss;the normal group was light red.Tongue and thin white moss are the main features.The tongue and tongue coating of the four groups of tongue features were statistically analyzed,P < 0.05.2.Colonoscopy features:Intestinal mirrors of colorectal cancer group showed intestinal mucosal bulge,erosion,surface with contaminated moss,narrow intestinal lumen,brittle,easy to hemorrhage,poor elasticity;polyp group colonoscopy more than polyps less than 0.5cm,Yamada II More common;the colonoscopy of the ulceration group showed diffuse hyperemia,edema,ulcer,erosion,mucosal granule thickening,and vascular texture blurred;the normal control group had a smooth lumen of the lumen and clear vascular texture.3.Tongue exfoliated cell maturity(MI,MV):Compared with the normal control group,the colorectal cancer group,the polyp group,and the ulceration group showed a significant decrease in the surface cells and a significant increase in the middle layer cells.The number of middle-level cells was in order from colorectal cancer group > polyp group >ulceration group > normal control group.In the MI distribution coordinates,the colorectal cancer group,the polyp group,and the ulceration group were parabolic,and the normal control group was a decreasing oblique line;the colorectal cancer group,the polyp group,the ulceration group,and the normal control group in MV showed an increasing trend.And statistical analysis,P <0.05.4.Detection of apoptosis-related proteins(EGFR,casepase-3,c-Jun):Compared with the other three groups,the positive expression rate of EGFR and c-Jun protein in the colorectal cancer group was statistically significant,P<0.05;the positive expression rate of casepase-3 protein was not statistically significant,P>0.05.Conclusions 1.In the characteristics of tongue image,from normal control group→stagnation group→ polyp group→colorectal cancer group,the color of tongue is gradually deepened from shallow to red,from red tongue to red tongue or dark red tongue→dark red tongue→purple tongue.There is a tendency of gradual aggravation of blood stasis,which may be caused by qi stagnation and blood stasis in the intestines,and the evil of dampness and heat is caused by fumigation on the tongue;from the normal control group → the ulceration group and the polyp group → the colorectal cancer group,the tongue coating is thin Gradually thicker,the greasy moss gradually increased,indicating that the phenomenon of dampness and heat stagnation has gradually increased.2.In the characteristics of colonoscopy,from the normal control group → polyp group → colorectal cancer group,the process of intestinal epithelial cells changes from normal epithelial to hyperplastic epithelium→ adenoma → adenocarcinoma,mucosal bulge gradually increases,and even intestinal The cavity is narrow and the mucosal surface is gradually roughened from normal to erosion.In the Kuijie group,there was no intestinal mucosal bulge,and the lesions showed diffuse congestion,edema,ulcers,erosion,mucosal granule thickening,and vascular texture blurred.3.In the maturity(MI,MV)of the exfoliated cells of the tongue,from the normal control group → the ulceration group → the polyp group → the colorectal cancer group,the MI value gradually increased,and the middle layer cells gradually increased,the surface cells gradually decreased,and the MV value.Gradually decreasing,suggesting the formation of yellow greasy moss,may be due to the gradual decrease of the number of cells in the middle layer to the surface cells,and the excessive accumulation of the middle layer cells.4.Detection of apoptosis-related proteins(EGFR,casepase-3,c-Jun):From the normal control group→the ulceration group→the polyp group→the colorectal cancer group,the EGFR and c-Jun proteins that promote cell proliferation showed a trend of high expression.It indicated that the exfoliated cells of the tongue coating were abnormally proliferated and the apoptosis was inhibited,suggesting that EGFR and c-Jun may play an important role in the process of intestinal malignant transformation.The rate of casepase-3,which promotes apoptosis,is low expression,but it cannot be used as an indicator to identify benign and malignant intestinal diseases.This may be indistinguishable from casepase-3 protein in performing casepase-3 and activation in the presence of zymogen forms.Casepase-3 related. |
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