Prevention And Treatment Of Fructus Broussonetiae On Drug-induced Liver Injury And Oxidative Stress Mechanism

Objectives: To observe the therapeutic effect of Fructus Broussonetiae on rats with drug-induced liver injury and to explore its possible mechanism of action.Methods: Sixty Wistar rats were randomly divided into normal group,model group,silybin control group,Fructus Broussonetiae high and low dose group,and acetaminophen was intragastrically administered and administered for 30 days.At the end of the experiment,blood was taken from the femoral artery to detect liver function and early markers of liver injury.The liver was dissected and the activity of oxidative damage index was detected.The pathological morphology was observed by HE staining.The expression of ROS1 was observed by immunohistochemistry.qRT-PCR was used to observe the expression changes of PPARαmRNA and PPARγmRNA.Results:Compared with the normal group,the hepatic lobular structure of the model group was broken,the hepatic cord arrangement was disordered,and the hepatic ballooning of the hepatocytes was accompanied by necrosis;the activities of ALT,AST,MDH,GLDH,ArgI,PNP and the contents of DBIL,TBIL,MDA in the model group were significantly increased(P<0.05 or P<0.01).the activities of SOD,GSH-Px and CAT and the content of GSH were decreased(P < 0.05 or P < 0.01);the expression of PPARαmRNA was significantly decreased(P < 0.01),the expression of PPARγmRNA was enhanced(P < 0.05 or P < 0.01);the expression of ROS1 was significantly enhanced(P < 0.01).Compared with the model group,each drug group showed local hepatocyte ballooning only around the central vein.;The activities of ALT,AST,GLDH,ArgI,PNP and the contents of DBIL,TBIL,MDA were decreased(P < 0.05 or P < 0.01),the activity of SOD,GSH-Px and the content of GSH were increased(P<0.05 or P<0.01);the expression of PPARαmRNA was increased(P < 0.05 or P < 0.01),and the expression of PPARγmRNA was decreased(P < 0.01).The expression of ROS1 was weakened(P<0.05 or P<0.01).Conclusions:1 Fructus Broussonetiae can reduce the levels of serum ALT,AST,DBIL,TBIL,GLDH,ArgI and PNP,improve the pathological damage of rat liver tissue,and has a good liver-protecting effect.2 Fructus Broussonetiae can enhance the activity of SOD,GSH-Px and the content of GSH in rat liver tissue,reduce the content of MDA,promote the expression of PPARαmRNA,and achieve the purpose of treating DILI by inhibiting oxidative stress.3 Fructus Broussonetiae can weaken the expression of ROS1 in liver tissue,inhibit the initiation and development of oxidative stress,and achieve the effect of preventing and treating DILI.