To Explore The Relationship Between The Family Of Multiple Tuberculosis And The Susceptibility To Tuberculosis

Objectives1.To explore environmental risk factors related to the incidence of tuberculosis in high-risk families with tuberculosis in Guangdong Province.2.To explore the relationship between the genetic variation of IFN-γ and IL-10 genes and the incidence of tuberculosis in high-risk families with tuberculosis in Guangdong Province.3.To explore the role of environmental-environment,gene-gene and gene-environment of IFN-γ and IL-10 genetic variation in the pathogenesis of tuberculosis in high-risk families with tuberculosis in Guangdong Province.Methods This study used a case-control study.A total of 262 subjects were included in 150 households,75 households in high-risk families with TB and 75 households with healthy families.Among them,there were 82 patients with tuberculosis,20 patients who had been cured,67 people with close contact with healthy families,and 93 patients in the control group.Through on-site epidemiological investigation,to explore the main environmental risk factors of TB in high-risk families with TB in Guangdong Province.The IFN-γ and IL-10 genes were genotyped by real-time PCR.Differences in genotype frequency and allele frequency distribution were performed using Fisher’s chi-square or Pearson chi-square test.Logistic regression was used to correlate genotypes and alleles with tuberculosis susceptibility.Interactions between environment-environment,gene-gene and gene-environment were usingmulti-factor dimensionality reduction(MDR)and the additive effect was analyzed by fork.Results1.Compared with HCs,the BMI below 18.5,primary school education and below,non-participating medical insurance and retired/unemployed in the tuberculosis case group accounted for 23.17%,74.39%,74.39% and 24.39%,respectively.The drinking,no gym and tuberculosis exposure in the tuberculosis case group accounted for 24.39%,73.17% and 85.37%,respectively.Living in rural areas,over one person/room in bedrooms,indoor lighting and indoor environmental sanitation in the tuberculosis case group accounted for 62.67%,85.33%,42.67% and 33.33%.After multi-factor analysis,the risk of illness in under primary school was 7.01 times compared with junior college and above(P=0.003,95% CI 2.46-20.02),and middle school was a secondary risk factor(OR=2.52,P=0.045,95%CI 1.02-6.26).The history of TB exposure was 12.73 times the risk of no history of TB exposure(P<0.001,95%CI 5.71-28.22).No gym were secondary risk factors,and 3.64 times of the risk of physical activity(P<0.001,95% CI 1.68-7.93).Living in rural areas were 5.90 times the risk of illness in urban areas(P<0.001,95%CI2.75-12.64).The poor of indoor environmental sanitation was 3.31 times the risk of the best environment(P = 0.002,95% CI 1.18-9.28).The AG+GG of IL-10 was 4.01 times the risk of AA,and the AG/G were the risk of AA\A(3.52,3.96 times),respectively.The AA of IFN-γ were 8.65 and 4.97 times the risk of tuberculosis infection by TT/AT+TT.The A of IFN-γ was 3.94 times the risk of tuberculosis infection.Haploid analysis,compared with AT haploid,AA haploid in tuberculosis risk was 3.22 times;GA haploid in tuberculosis risk was 3.65 times.According to MDR analysis,there were high-risk combinations of infected tuberculosis: under primary school and history of TB exposure;the AA of IFN-γ and AA of IL-10,the TT of IFN-γ and AG of IL-10;under primary school,history of TB exposure with the AA/AT of IFN-γ.2.Compared with HHC,the incidence of tuberculosis with smoking wasfound to be 26.83%.After multi-factor analysis,the males were the main risk factor for tuberculosis,which was 3.50 times the risk of women.The AG+GG of IL-10 was 7.38 times of the risk of tuberculosis infection with AA.Compared to the GG+AA,AA and A,the AG/G were 5.85,6.16 and 6.77 times the risk of tuberculosis infection.According to MDR analysis,male,smoking and the AG/GG of IL-10 would have a higher risk of tuberculosis infection.Conclusions1.Compared to HCs,it was found that under primary school,no gym,drinking,history of TB exposure,in rural areas and the poor of indoor environmental sanitation may be risk factors for high-risk families with TB.Contrasted to HHC,it found that males and smoking may be risk factors for high-risk families with TB.2.Regardless of the HCs or HHC,the AG genotype and G allele of the1082G/A of IL-10 gene may be candidates for tuberculosis in the high-risk family of TB in Guangdong Province.3.Compared to HCs,the under primary school and history TB exposure;the AA of IFN-γ and AA of IL-10,the TT of IFN-γ and AG of IL-10;under primary school,history of TB exposure with the AA/AT of IFN-γ had a higher risk of tuberculosis infection.Compared to HHC,males,smoking and the AG/GG of IL-10 would have a higher risk of tuberculosis infection.