Prognosis Analysis Of Prostate Cancer And Mechanism Research Of TSPAN1 In Disease Progression

Purposes:To explore the prognosis factors of prostate cancer.In order to distinguish patients resistant to first-line treatments in time,and pave the way to personalized therapy.Substances and methods:Find out prognostic related clinical variables by survival analysis.Using cox regression to determine the independent predictive value of the prognostic relevant variables.Through searching the published databases,looking for prognostic relevant abnormal gene expression.And confirm the result by immunohistochemical staining in our prostate tumor tissues.Knockdown and overexpression the target gene in prostate cancer cell lines,and observe the changes of cell proliferation and migration.Explore the mechanisms by western blot and flow cytometry.Results: For metastatic prostate cancer patients receiving hormonal treatment,Gleason score,PSA nadir and time to PSA nadir are independent prognosis factors of CRPC.For localized prostate cancer patients receiving radical prostatectomy,preoperative PSA,Gleason score and TSPAN1 expression level are related to biochemical recurrence.TSPAN1 expression is an independent prognostic factor of biochemical recurrence.Low expression of TSPAN1 is related to invasive prostate cancer in PTEN/P53 deletion mouse model.TSPAN1 Knockdown in vitro can activate PI3K/Akt pathway,and is related to obvious cell cycle transition from G1 to S phase and promote cell proliferation and migration.Conclusions: Bone metastatic prostate cancer with high Gleason score,high PSA nadir or short interval time to nadir are more likely to have CRPC early.TSPAN1 could be used as an independent prognosis factor in predicting early biochemical recurrence after radical prostatectomy.The interaction between TSPAN1 and PI3K/Akt pathway may contribute to disease progression.