Study On Antiviral Activity Of Herbal Ingredient A Against Hepatitis C Virus (HCV) Base On Huh7.5 Cell Model

C hepatitis is a seriously infectious disease in the worldwide,which is caused by Hepatitis C Virus(HCV).As no effective vaccine,pegylated interferon-α(IFN-α)and ribavirin are applied for the basic treatment of the C hepatitis.But the treatment effects are limited as the diversity of the viral genotypes.Traditional Chinese medicine formulas have a long history of use in the prevention and treatment of diseases.In this study,we developed a model for screening the anti-HCV bioactive compounds in vitro.Compound A that is selected from the compound libraries of natural medicine can significantly inhibit the activity of HCV virus.The IC50 of compound A is 6.34μM by the dose-effect relationship.The inhibition of the viral replication related with the concentration of compound A with the accordance of EGFP report system.Besides,the compound A is nontoxic on cells within the effective concentration,which initially confirmed the safety of the compound.Furthermore,it is found that the compound A inhibited NS3 protein serine protease and helicase activity by detecting the inhibition rate of HCV non-structural proteins NS3 protein.Thus,the compound A inhibits the translation processing and replication of HCV in cells.In summary,this study established a highly effective method of screening anti-HCV compounds and find a safe and effective antiviral compound A at the cellular level.It also elucidates the effective mechanism of the compound with HCV.It is significant for the prevention and treatment of chronic hepatitis C disease by traditional Chinese medicine.