| Objective Epstein-Barr virus(EBV)is a kind of lymphotropic herpes virus.Its primary infection can lead to infectious mononucleosis,and is also related to various malignant tumors and immune diseases.EBV is mainly transmitted through saliva,and can also infect susceptible people through blood transfusion and organ transplantation.The purpose of this study is to understand the viremia rate of EBV in clinical use of blood and the genotyping of infected blood products in Hohhot,and to discuss whether it is necessary to include EBV screening in the evaluation criteria of qualified blood donors as the routine detection of HBV,HCV,HIV and TP,so as to reduce the possibility of EBV infection through blood transfusion and further clarify the significance of prevention and treatment of EBV infection.Methods(1)Leukocyte-depleted suspension red blood cells were collected from blood donors whose screening results of HBV,HCV,HIV and TP were negative from August 2016 to February 2017 in Hohhot,Inner Mongolia.Blood samples were processed and stored in accordance with Measures for the “Administration of Clinical Blood Use in Medical Institutions”.500 cases were randomly selected from the samples.Braids in blood bags were taken as test samples to prepare peripheral blood mononuclear cells(PBMC)samples.PCR was used for quantitative detection of EBV nucleic acid in samples,and the number and proportion of samples were calculated respectively according to the different viral loads.(2)According to the linkage polymorphism of EBNA2 and EBNA3 C gene and the retention and deletion of some fragments of BamHI gene,EBV positive samples were typed by A/B,F/f and C/D by PCR combined with restriction fragment length polymorphism detection.The number and proportion of other samples of each type were calculated respectively,and the main types of clinical blood products infected with EBV are counted.Results(1)44 out of 500 randomly selected PBMC isolated from qualified clinical blood were positive for EBV DNA,with a positive rate of 8.8%(44/500).Among 44 EBV-DNA positive blood samples,according to different viral loads,we got the following data: 37 cases were 5.0E+02-1.0E+03copies/ml.4 cases were 1.0E+03-1.0E+04copies/ml.2 cases were 1.0E+04-1.0E+05copies/ml.1 case was 1.0E+05-1.0E+06 copies/ml.(2)44 EBV DNA positive specimens were further typed.The detection rates of A/B typing were as follows: Type A was 36.36%(A6/44)and type B was 63.64%(28/44).The detection rates of F/f typing were as follows: Type F was 79.55%(35/44)and type f was 20.45%(9/44).The detection rates of C/D typing were as follows: Type C was 70.45%(31/44)and type D was 29.55%(13/44).Conclusion(1)Blood transfusion can lead to EBV infection.The detection rate of EBV viremia in blood for clinical use in Hohhot is 8.8%.(2)The majority of EBV positive samples are type B,type F and type C EBV infection.(3)It is suggested that blood donors should be routinely tested for EBV DNA carrying to reduce the incidence of infectious diseases caused by blood transfusion. |
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