Study On The Relationship Between Vaginal Microecological Changes And HPV Infection And Cervical Lesions In Postmenopausal Women

Objective To explore the relationship between vaginal microecology and HPV infection in postmenopausal women and the characteristics of HPV infection types in postmenopausal women by detecting vaginal microecology and HPV type in healthy postmenopausal women and postmenopausal cervical lesions.Methods 100 postmenopausal low-grade cervical lesions(CIN I),100 high-grade cervical lesions(CIN II,III)and 30 cervical squamouscell carcinomas were selected as the study group and 100 healthy postmenopausal women as the control group.Vaginal microecology of vaginal secretions was detected by dry chemical enzymatic method and HPV genotyping was detected by reverse dot hybridization of PCR.Result:(1)The age of menopause,the number of pregnancies and the number of births in four groups had statistical significance.The age,menopausal years,the number of pregnancies and the number of births in HSIL group and squamous cell carcinoma group were higher than those in control group and LSIL group,and the difference was statistically significant;(2)The distribution of HPV infection types in different grades of cervical lesions after menopause had statistical significance(P < 0.05);with the increase of the grade of lesions,the high-risk HPV infection type The proportion of V infection and multiple infection increased(P < 0.05).There was no significant difference in the positive rate of high-risk HPV between HSIL group and squamous cell carcinoma group(P > 0.05);(3)There was significant difference in vaginal PH among groups of postmenopausal cervical lesions at different levels;(4)There was significant difference in the abnormal rate of Lactobacillus among groups of postmenopausal cervical lesions(P < 0.05).With the development of CIN,the abnormal rate of Lactobacillus increased;(5)The positive rate of hydrogen peroxide in each group had significant difference(P < 0.05),the positive rate of hydrogen peroxide decreased,the ability of Lactobacillus to produce hydrogen peroxide decreased,and promoted the development of CIN.(6)There were significantdifferences in the classification of bacterial flora density in each group(P < 0.05),among which there were significant differences in the abnormal rate of LSIL,HSIL and squamous cell carcinoma groups and menopausal control group(P < 0.05),along with CIN.The abnormal rate of bacterial flora density increased with the development of cervical lesions.(7)The distribution of dominant bacterial flora in different grades of cervical lesions after menopause had statistical significance(P <0.05).With the increase of pathological grade,the number of normal bacteria(G+b(L))decreased,the proportion of G-b(s)increased,and the proportion of bacterial inhibition increased.There were significant differences among the groups(P<0.05).Conclusion(1)Postmenopausal HPV infection,especially persistent infection,is a risk factor for postmenopausal cervical lesions,and the incidence of cervical cancer will increase with the increase of lesion grade and risk.(2)HPV high-risk type,dominant bacteria,Lactobacillus grading,hydrogen peroxide and cervical lesion progress of the correlation coefficient is larger,strong correlation.Abnormal Lactobacillus,BV,negative hydrogen peroxide and decrease of dominant bacteria are risk factors for the occurrence and development of postmenopausal cervical lesions.(3)For postmenopausal women,attention to vaginal microecological balance and regular screening can prevent HPV infection in postmenopausal women and reduce the development rate of lesions.