| Objectives To explore the expression levels of miR-22 and PTEN in epithelial ovarian cancer tissues,and analyse the relation between the expression levels and the clinical features.Furthermore,to investigate the effect of miR-22 expression on proliferation and migration of ovarian cancer cells and the possible mechanism.Methods 1 From November 2017 to August 2018,40 resected epithelial ovarian cancer tissue samples,32 benign ovarian tumor samples,and 30 normal ovarian tissue samples in Tangshan Gongren Hospital Affiliated to North China University of Science and Technology were collected respectively.Quantitative real-time PCR(qRT-PCR)were used to detect the expression levels of miR-22 mRNA,and western blot were used to detect the expression levels of PTEN protein.The expression levels of miR-22 mRNA and PTEN protein,the correlations in epithelial ovarian cancer tissue samples of different age,FIGO stages,pathological grades,and histological types,lymph node metastasis,ascites were analyzed.Pearson Correlation Coefficient was used to analyse the correlation between the expression levels of miR-22 mRNA and PTEN protein.2 Human ovarian cancer SKOV3 cells were cultured in vitro,and SKOV3 cells which over-expressed miR-22 were constructed by using cell transfection technique.3 The proliferation ability of each group was detected by CCK-8 test;the migration ability of each group was detected by scratch test;the expression of PTEN mRNA in each group was detected by qRT-PCR,and the expression level of PTEN protein in each group was detected by western blot.Results 1 The expression of miR-22 in epithelial ovarian cancer tissues(0.587±0.035)was lower than that in normal ovarian tissues(0.993±0.053)and benign ovarian tumor tissues(0.975±0.058),the difference was statistically significant(F=815.491,P<0.05).The expression level of miR-22 was not significantly different between benign ovarian tumor tissues and normal ovarian tissue(P>0.05).The expression of PTEN protein in epithelial ovarian cancer tissues(0.266±0.029)was also lower than that in normal ovarian tissues(0.730±0.027)and benign ovarian tumor tissues(0.719±0.021),the difference was statistically significant(F=3676.201,P<0.05).The expression level of PTEN was not significantly different between benign ovarian tumor tissues and normal ovarian tissue(P>0.05).2 The expression of miR-22 in epithelial ovarian cancer tissues decreased gradually with the progression of FIGO stage and pathological grade(P<0.05),and PTEN protein expression decreased with the FIGO stage,pathological grade and lymph node metastasis(P<0.05).With the decrease of miR-22,PTEN protein decreased too,and the expression of miR-22 was positively correlated with that of PTEN protein(r=0.867,P<0.05).3 Compared with the blank control group(0.720±0.009)and miR-NC group(0.715±0.009),cell proliferation ability of the miR-22 mimics group(0.516±0.005)was significantly lower than other groups(F= 619.687,P<0.05),while there was no significant difference between the blank control group and the miR-NC group(P>0.05).4 Compared with the blank control group(48.92%±0.04%)and miR-NC group(45.78%±2.49%),the wound healing percentage in the miR-22 mimics group(11.83%±0.66%)was significantly decreased(F=176.147,P<0.05);while there was no significant difference in scratch area between the blank control group and the miR-NC group(P>0.05).5 In the miR-22 mimics group(6.165±0.073),the expression level of PTEN mRNA was significantly higher than that in the blank control group(1.005±0.007)and the miR-NC group(1.067±0.029)(F=12870.441,P<0.05),while there was no significant difference in PTEN mRNA expression levels between the blank control group and the miR-NC group(P>0.05).6 Compared with the blank control group(0.198±0.014)and the miR-NC group(0.208±0.006),the expression of PTEN protein in the miR-22 mimics group(0.599±0.069)was significantly increased(F=93.276,P<0.05);and the expression of PTEN protein was not significantly different between the blank control group and miR-NC group cells(P>0.05).Conclusions 1 The expressions of miR-22 and PTEN was down-regulated in epithelial ovarian cancer tissues.2 With the progression of the disease,the expressions of miR-22 and PTEN decreased gradually,and the expression of miR-22 was positively correlated with that of PTEN.3 In SKOV3 cells,the expression of miR-22 could up-regulate the expression of PTEN in SKOV3 cells,inhibiting the proliferation and migration of ovarian cancer cells.Thus,miR-22 and PTEN could participate in the occurrence and development of ovarian cancer.Figure13;Table11;Reference 56... |
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