The Role Of Microbiota On The Development Of Non-small-cell Lung Cancer

Objective: To characterized gut and lung microbiome in patients with Non-small-cell lung cancer(NSCLC),and explored the effects of antibiotic treatment on Lewis cell line growth in the xenograft model and brain metastasis model.Methods: We collected 102 fecal and 71 sputum samples from Wuhan Union Hospital.The Illumine Miseq sequencing platform was used to analyze 16 S rRNA variable regions V3 and V4 in these samples.The role of antibiotic treatment was subsequently investigated both in xenograft model and brain metastases model.Results:(1)The sputum microbial diversity of control-S group was higher than NSCLC-S.The microflora structure of control-S and NSCLC-F group was significantly different.KEGG pathway analysis showed that signaling pathways involved in bladder cancer was enriched in NSCLC-S group.(2)However,no difference in diversity was showed between the fecal microbial of Control-F group and NSCLC-F group.There were some differences in the microflora structure between control-F and NSCLC group.KEGG pathway analysis showed that MAPK signaling pathway was enriched in NSCLC-F group.(3)The fecal microbial alpha diversity was similar between BM(+)group and BM(-)group.But there were some differences in the microflora structure between the 2 groups.The OTU2412 was abundant in BM(-)group samples and exhibited moderate classification potential.(4)An impaired microbiota of mouse,antibiotic treatment,promoted tumorigenesis in subcutaneous xenograft,but inhibited tumorigenesis in brain metastasis.Impaired microbiota at least partially influenced Lewis progression through acute and chronic inflammation of the intestine.Conclusion: It suggested that there were some differences in the microflora structure of the fecal and sputum samples of lung cancer patients.Antibiotic treatment at least partially influenced Lewis progression through acute and chronic inflammation of the intestine.