Vitamin D Inhibits The Proliferation Of Gastric Cancer Cells Via Down-regulating The Expression Of β-catenin Phosphorylation By VDR

Objective:To investigate the effect and molecular mechanism of vitamin D（VD）and vitamin D receptor（VDR）on the proliferation of gastric cancer cells.Methods:First,we observed the expression of VDR in SGC-7901 and MKN-45 gastric cancer cells by immunofluorescence assay.Furthermore,VDR-shRNA stable cell lines of SGC-7901 and MKN-45 cells were selected by puromycin under using shRNA interfere with lentivirus and transfecting the gastric cancer cells.The effects of VD/VDR on the proliferation and cell cycle of two kinds of gastric cancer cells were detected by CCK-8（Cell Counting Kit-8）assay,colony formation assay,flow cytometry and Western blot.Eventually,the effects of VD/VDR on the expression ofβ-catenin phosphorylation（p-β-catenin）of gastric cancer cells were tested by Western blot.Results:VDR was expressed in both MKN-45 and SGC-7901cells.The proliferation and colony formation of gastric cancer cells were significantly inhibited,while the expression of p-β-catenin was inhibited in the presence of Osteotriol（1α,25（OH）₂D₃）,but there were no significant effects on cell cycle distribution and the expression of cyclin D1.After down-regulation the expression of VDR,VD had no significant effect on the the expression of p-β-catenin.Conclusion:VDR can inhibit the proliferation of gastric cancer cells via inhibiting the expression of p-β-catenin.