Clinical Value Of CfDNA Total Concentration And Long Fragment DNA Concentration In Peripheral Venous Blood Of Patients With Operative Non-small Cell Lung Cancer

Malignant tumors have become one of the major diseases that seriously threaten human health.According to the cancer statistics 2019,which was released by National Cancer Center,the deaths of malignant tumors account for 23.91% of the total deaths of the residents.The incidence and mortality of lung cancer are malignant in China.The tumors ranked first,and the pathological types of lung cancer were mostly non-small cell lung cancer(NSCLC),accounting for 80%-85% of the total number of lung cancers.With the improvement of medical level in China,lung cancer has entered a new era of comprehensive treatment such as surgery,radiotherapy,chemotherapy,molecular targeted therapy and immunotherapy,but the overall survival rate is still not ideal.The survival rate of lung cancer patients in different stages is very different.In patients with large,non-small cell lung cancer,the 5-year survival rate is only 5% in the case of distant metastasis.In recent years,with the development of tumor cytogenetics,the value of circulating free-DNA(cfDNA)in the diagnosis,evaluation and prognosis of tumors has become a hot topic.Many studies at home and abroad have shown that the cfDNA level of patients with malignant tumors is significantly higher than that of normal people.The cfDNA levels of breast cancer,gastric cancer and colorectal cancer patients are positively correlated with the size of tumor volume in vivo.Can we detect the level of cfDNA in non-small cell lung cancer patients and compare it with healthy people to reflect the change of tumor burden(Tumor Burden,TB)in patients with non-small cell lung cancer,and then use it for the auxiliary diagnosis of patients with non-small cell lung cancer,Efficacy assessment and monitoring of recurrence or metastasis are worth exploring.Objective:This study is to investigate the correlation between cfDNA and long-segment DNA concentration in peripheral blood of patients with non-small cell lung cancer and bioinformatics analysis,and to explore its correlation with TB status in patients with non-small cell lung cancer.Researching the clinical value of patient-assisted diagnosis,efficacy evaluation,and monitoring of recurrence or metastasis in the NSCLC.Methods:Sixty patients with operable non-small cell lung cancer admitted to the Department of Thoracic Surgery,Chengde Central Hospital from January 2017 to June 2018 were enrolled in the experimental group.The peripherals were collected 1 day before surgery,7 days after surgery,and 20 days after surgery.Venous blood samples were collected from the physical examination center of Chengde Central Hospital in the same period.60 healthy people with comparable age and gender were selected as the control group.Peripheral venous blood samples were collected and LightCycler 480 Swiss Roche LC480 real-time quantitative PCR(QPCR)was applied.The total concentration of cfDNA and the concentration of long-segment DNA in the specimens were quantitatively detected,and the results were statistically analyzed using SPSS 22.0 software.Results:1.In the experimental group,the total concentration of cfDNA in peripheral venous blood was 15.11±5.26ng/ml one day before operation,and the long-segment DNA concentration was 2.28±1.27ng/ml,which was significantly higher than the total concentration of cfDNA in the control group 8.03±2.35ng/ml and long.The DNA concentration of the fragment was 0.71±0.22ng/ml,and the difference was statistically significant(P<0.01).2.In the experimental group,the total concentration of cfDNA in peripheral venous blood was 15.20±3.81ng/ml,the long-segment DNA concentration was 2.36±1.36ng/ml,and the total concentration of cfDNA was 15.11±5.26ng/ml and long fragment before surgery.There was no significant change in DNA concentration of 2.28±1.27ng/ml,and the difference was not statistically significant(P>0.05).3.In the experimental group,the total concentration of cfDNA in the peripheral venous blood was 13.98±3.19ng/ml,and the long-segment DNA concentration was 1.93±1.10ng/ml.The total concentration of cfDNA was 15.11±5.26ng/ml and the long fragment was 1 day before operation.The DNA concentration was significantly decreased by 2.28±1.27ng/ml,and the difference was statistically significant(P<0.05).4.In the experimental group,the total concentration of cfDNA in the peripheral venous blood was 13.98±3.19ng/ml,and the long-segment DNA concentration was 1.93±1.10ng/ml.The total concentration of cfDNA in the peripheral venous blood was 15.20±3.81 ng.The concentration of /ml and long fragment DNA was significantly decreased by 2.36±1.36ng/ml,and the difference was statistically significant(P<0.05).Conclusion:1.The total concentration of cfDNA and long-segment DNA in peripheral venous blood of patients with non-small cell lung cancer was significantly higher than that of healthy people 1 day before surgery,indicating that the level of cfDNA in peripheral venous blood of patients with malignant tumors was significantly increased,which helped to assist non-small cells.This could help to assist early diagnosis of the NSCLC.2.The total concentration of cfDNA and long-segment DNA concentration in peripheral venous blood of patients with non-small cell lung cancer did not change significantly compared with 1 day before surgery.This is in contradiction with the reduction of tumor burden in patients after surgery.Therefore,cfDNA was detected at this time.The total concentration and long-segment DNA concentration did not accurately reflect the changes of tumor burden in patients.3.The total concentration of cfDNA and the concentration of long-segment DNA in peripheral venous blood of patients with non-small cell lung cancer were significantly lower than those before surgery,which was consistent with the decrease of tumor burden in patients after surgery.Therefore,the total concentration of cfDNA was detected at this time.And long fragment DNA concentration can better reflect the changes of tumor burden in patients.4.The total concentration of cfDNA and long-segment DNA concentration in peripheral venous blood of patients with non-small cell lung cancer were significantly lower than that of 7 days after surgery,indicating that the patient gradually recovered from the surgical trauma at 20 days after surgery,and the body environment became stable.At this time,the detection of the total concentration of cfDNA and the concentration of long-segment DNA can better reflect the changes of tumor burden in patients.