TRPM3 Induces Epithelial-mesenchymal Transition Via The Wnt/β-catenin Signaling Pathway In Epithelial Ovarian Cancer

Transient receptor ion channels 3(TRPM3),as a member of the family of trransient receptor potential ion channel(TRP)cation channels in the cell membrane.And TRPM3 is involved in the important physiological functions in many tissues and cells.However,it is still lacking in the biological function and mechanism of TRPM3 in epithelial ovarian cancer(EOC).Therefore,we hope to determine whether TRPM3 involved in the invasion and metastasis of epithelial ovarian cancer and to further explore its molecular biological mechanism in this thesis.In our study,compared with normal ovarian tissue,TRPM3 in epithelial ovarian cancer cells were overexpressed obviously.In this paper,the TRPM3 gene in epithelial ovarian cancer cells Hey and SKOV3 were knocked out by small interfering RNA,then Western Blot assay showed that the expression of E-cadherin was increased,while the expression of N-cadherin and Snail was decreased.Furthermore,the invasion and migration ability of EOC cells decreased significantly.cell migration and invasion abilities decreased by Transwell experiment and scratch migration assays.Epithelial-mesenchymal transition(EMT)plays a key role in the invasion and metastasis of many malignant tumors.It can regulate many signaling pathways,such as Wnt/β-catenin signaling pathway.Furthermore,TRPM3 can up-regulate the expression level of EMT markers,such as N-cadherin and Snail,which were restored via silencing TRPM3 expression.The results showed that the silence of TRPM3 expression would result in Wnt/β-catenin signaling pathway related genes β-catenin and Cyclin D1 expression decreased obviously.At the same time,after adding the Wnt/ β-catenin signal pathway inhibitor XAV939,the expression of EMT-related proteins and Wnt/β-catenin signal pathway related proteins(E-cadherin,N-cadherin,Snail,β-catenin,CyclinD1)in the interference group and the control group decreased.So we think TRPM3 can promotes EMT by activating Wnt/beta-catenin signaling pathway,thus causing invasion of EOC cells.Thus,TRPM3 may become a new target in epithelial ovarian cancer.