Effect Of Mechano Growth Factor On Levator Ani Muscle Repair In Patients With Stress Urinary Incontinence

Objective:To explore the specific mechanism of the therapeutic effect of MGF on patients with SUI through detecting the survival rate of levator ani myoblasts and the expression of Laminin and Dystrophin in female patients with SUI.Methods:Levator ani myoblasts were isolated from female patients with SUI and patients undergoing radical resection of rectal cancer,and identified by sarcomeric actin immunofluorescence assay.The survival rate of levator ani myoblasts in patients with SUI treated with different concentrations of MGF(50 ng/mL、100 ng/mL、150 ng/mL、200 ng/mL)at different time(24 h,48 h,72 h,96 h)was measured by MTT method.After 72 hours incubation of myoblasts,the contents of these two proteins were determined by Western Blot.Results:1.The effects of different concentrations of MGF on the proliferation of levator ani myoblasts in patients with SUI were different(P < 0.05);The effects of different action time of MGF on the proliferation of levator ani myoblasts in patients with SUI were different(P < 0.05);There was interaction between MGF concentration and MGF action time.When MGF concentration was 150 ng/mL and MGF action time was 72 h,the survival rate of levator ani myoblasts in patients with SUI was the highest(P < 0.05).2.Compared with the normal control group,the expression of Laminin andDystrophin in levator ani myoblasts in SUI group was lower(P<0.05);Compared with the SUI group,the expression of Laminin and Dystrophin in levator ani myoblasts in MGF group(150 ng/mL)was higher(P<0.05).Conclusion:1.The concentration of MGF and the action time of MGF can interact with each other to promote the proliferation of levator ani myoblasts in SUI patients cultured in vitro.2.MGF can promote the expression of Laminin and Dystrophin in levator ani myoblasts of SUI patients.