The Study On The Expression Of DAB2IP In Human Eastric Cancer And The Role Of DAB2IP In The Proliferation And Metastasis Of Human Gastric Cancer Cells

[Objective]To investigate the expression of DAB2IP in gastric cancer tissues and its correlation with clinicopathological features of gastric cancer patients;To determine the effect of D AB2IP downregulation on the proliferation and metastasis of gastric cancer cells.[Methods]Immunohistochemistry(lHC)was used to detect the expression of DAB2IP in 83 paired gastric cancer tissues and adjacent normal tissues,and the relationship between DAB2IP expression and clinicpathological features of patients with gastric cancer was analyzed;Western blot and QRT-PCR were used to detect the expression level of DAB2IP in four gastric cancer cells and to select the cell line with high level of DAB2IP;Lentivirus transfection technology was used to establish cell line in which DAB2IP was stably downregulated;The effects of DAB2IP knockdown on the proliferation and metastasis of gastric cancer cells were evaluated by colony formation assay and cell migration assay;Western blot and QRT-PCR were used to detect the influence of DAB2IP knockdown on EMT in gastric cancer cells.[Results]Compared with adjacent normal tissues,DAB2IP expression in human gastric cancer tissues was significantly downregulated,and its expression level was associated with tumor size,histological differentiation,depth of invasion,lymph node metastasis and TNM stages;AGS gastric cancer cells expressed higher DAB2IP in both protein and mRNA level;The DAB2IP knockdown efficiency was high in AGS cells;Silencing DAB2IP enhanced the proliferation and migration of AGS cells;Silencing DAB2IP reduced E-cadherin expression but increased Vimentin expression at both protein and mRNA levels.[Conclusions]DAB2IP expression in gastric cancer tissue was significantly downregulated compared to adjacent normal tissues and its expression level was associated with tumor size,histological differentiation,depth of invasion,lymph node metastasis and TNM stages,indicating DAB2IP plays an important role in the development and progression of gastric cancer;DAB2IP knockdown promoted the proliferation and metastasis of gastric cancer cells,suggesting that DAB2IP is involved in regulating gastric cancer cell proliferation and metastasis;DAB2IP knockdown promoted EMT progress in gastric cancer cells,suggesting that DAB2IP may regulate gastric cancer cell migration by modulating the EMT progress.