Bergenin Activates SIRT1 As A Novel Therapeutic Agent For Osteogenesis Of Bone Mesenchymal Stem Cells

Objective:To explore the effect of bergenin on osteogenic differentiation of mesenchymal stem cellsMethods:Human mesenchymal stem cells were obtained,which were divided into the brothin group and the bergenin group.Both osteogenic differentiation and adipogenic differentiation were performed.The effects of bergenin on osteogenic differentiation and adipogenic differentiation of mesenchymal stem cells in vivo were determined by staining and detecting osteogenic and adipogenic specific markers.Twelve healthy male SD rats were taken.Rats were randomized into two groups:experimental group(n=6)and control group(sham operation group n=6).A 4 mm critical dimension defect was created in the parietal bone using a trephine.The experimental group was intraperitoneally injected with bergenin in 50 mg/kg body weight of phosphate buffered saline(PBS),and the sham operation group was treated with an equal amount of PBS per week.Rats were sacrificed in a CO 2 chamber 8 weeks after surgery.Collect skull for radiographic and histological analysis and assess serum ALP activityResults:After pretreatment with 1-100 μM bergenin,the osteogenic-specific markers ALP,RUNX2,COL1A1 and SIRT1 mRNA levels were significantly increased on day 3 and day 5 of BMSC in the experimental group compared with the control group(P<0.05).Western blot showed that the expression levels of RUNX2,COL1A1 and SIRT1 were significantly increased on the 3rd and 5th day of BMSC in the experimental group,and the expression of the adipogenic specific marker PPARy protein was decreased(P<0.05).Alizarin red staining showed a significant increase in calcium deposition in the 1-100-μM bergenin treated group.Oil red staining showed a significant reduction in fat droplets in the bergenin treated groupMicro-CT analysis showed an increase in bone formation in the skull defect model of the Berginin group of rats in the Berginin group compared with the sham-operated group 8 weeks after surgery.In addition,higher levels of ALP activity were detected in the serum of the Bergenin group.Conclusion:The results suggest that bergenin can promote the osteogenic differentiation of BMSCs and inhibit the adipogenic differentiation of bone marrow mesenchymal stem cells.It has a significant promoting effect on the repair of rat bone defects.