| Objective:Establishment of a rat model of vascular cognitive dysfunction(VCI)with modified bilateral common carotid artery ligation(modified 2-V0 method),and through the lateral ventricle injection of Sulfiredoxin1(Srxn1)and nuclear transcription factor E2 related factor 2(Nrf2)gene interference fragment,the Morris water maze test was then used to evaluate the behavior of the rat model of vascular cognitive dysfunction.Methods:1.66 male Sprague-Dawley rats were randomly divided into blank control group(group A),sham operation group(group B),routine operation group(group C),Srxn1-siRNA operation group(group D),and Nrf2-siRNA operation group(Group E)2.Group A did not receive any treatment.Group B only separated bilateral common carotid arteries.Group C used modified 2-V0 method to establish a rat model of vascular cognitive dysfunction.In group D,Srxn1 gene interference fragment was injected into the lateral ventricle of rats 24 hours before model establishment.In group E,Nrf2 gene interference fragment was injected into the lateral ventricle of rats 24 hours before modeling3.After 30 days of modeling,Morris water maze test was used to evaluate the behavior of each group of rats.In the navigation experiment,the escape latency of each group was recorded.In the space exploration experiment,the number of platform crossings,the residence time of the platform area,and the residence time of each quadrant were recorded in each group of rats.Results:1.Construction of animal models:A total of 8 rats died in this experiment,and 46 rats were successfully screened by Morris water maze test.The overall success rate of rat model of vascular cognitive dysfunction was 88.5%.2.In the Morris water maze positioning experiment,compared with the blank control group,there was no significant difference in the escape latency between the sham operation group(P>0.05),and the escape latency of the rats in the conventional operation group was prolonged,the difference was statistically significant(P<0.05).Compared with the conventional surgery group,the escape latency of the rats in the Srxn1-siRNA group and the Nrf2-siRNA group was prolonged,and the difference was statistically significant(P<0.05).There was no significant difference in the escape latency between the Srxn1-siRNA group and the escape latency of the Nrf2-siRNA group(P>0.05).3.In the Morris water maze space exploration experiment,compared with the blank control group,there was no significant difference in the number of platform crossing times and platform quadrant residence time between the sham operation group(P>0.05),in the routine operation group,the number of platform crossings per unit time decreased(P<0.01),and the resting time in the platform area decreased(P<0.01),the difference was statistically significant.Compared with the conventional surgery group,the number of platform crossings per unit time in the Srxn1-siRNA operation group and the Nrf2-siRNA operation group decreased(P<0.05),and the platform area residence time decreased(P<0.05),the difference was statistically significant.There was no significant difference in the number of platform crossings and the resting time of the platform in the Srxn1-siRNA group and the Nrf2-siRNA group(P>0.05).4.In the space exploration experiment of Morris water maze,compared with the blank control group,there was no significant difference in the residence time of each sham operation group,routine operation group,Srxn1-siRNA operation group and Nrf2-siRNA operation group,which was not statistically significant(P> 0.05).Conclusion:A modified bilateral common carotid artery ligation(modified 2-V0 method)can successfully establish a rat model of vascular cognitive dysfunction,and confirmed that this model can be used in the next experiment. |
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