| Background:SLCO4A1 antisense RNA 1(SLCO4A1-AS1)was an up-regulated expression of long non-coding RNA revealed by TCGA database.In this study,we aim to investigate expression,prognosis and biological function of SLCO4A1-AS1 in colorectal cancer.Methods:The expression levels of SLCO4A1-AS 1 in various tumors were analyzed through downloading data from TCGA and GTEx databases,and then combined with the clinical information in the TCGA database,the relationship between the expression level of SLCO4A1-AS1 and the prognosis of patients with colorectal cancer was analyzed;Collecting clinical information on 109 patients with colorectal cancer and their tumor samples and paired normal samples,we measured the expression levels of SLCO4A1-AS1 in 109 paired patients tissues using quantitative RT-PCR assay(qRT-PCR),and then analyzed its association with clinical case factors by Pearson x2 test;Kaplan-Meier survival analysis were used to analyze the correlation between SLCO4A1-AS1 expression and prognosis in patients with colorectal cancer;qRT-PCR was used to detect the expression of SLCO4A1-AS1 in colorectal cancer cells and human normal colon cells;SLCO4A1-AS1 overexpression and knockdown plasmids were designed,and their overexpression and knockdown efficiency were detected;SLCO4A1-AS1 overexpression and knockdown stable strains were constructed;The effect of SLCO4A1-AS1 overexpression and knockdown on cell proliferation of colorectal cancer was measured by CCK-8 and colony formation assay;The effect of overexpression and knockdown of SLCO4A1-AS1 on cell cycle and apoptosis was checked by flow cytometry and apoptosis assay;The effect of SLCO4A1-AS1 overexpression and knockdown on cell migration ability was detected by scratch test;Tumor formation in nude mice was used to detect the effect of SLCO4A1-AS1 on tumorigenic ability in vivo.Results:We found that SLCO4A1-AS1 was differentially expressed in a variety of tumors,and significantly elevated(log2FC=3.61,P<0.001)in colorectal cancer by analyzing TCGA and GTEx databases.The TCGA database also indicated that the colorectal cancer patients with high SLCO4A1-AS1 expression had a shorter overall survival rate(P<0.0378).The expression of SLCO4A1-AS1 was analyzed in 109 cases of colorectal cancer clinical tissue samples,and the results showed that SLCO4A1-AS1 was up-regulated more than 1.5-fold in 72.1%(79/109)colorectal cancer tissues,and the up-regulation of SLCO4A1-AS1 was correlated with lymphatic invasion(P=0.022),advanced tumor stage(P=0.038),shorter overall survival rate(log rank=8.391,P=0.0038)and disease-free survival time(log rank=4.025,P=0.0448)in colorectal cancer patients.Univariate and multivariate Cox regression models showed that expression of SLCO4A1-AS 1 was an independent prognostic factor for colorectal cancer(HR=2.397,95%CI:1.025-5.602,P=0.044);functional gain and loss experiments showed up-regulated SLCO4A1-AS1 expression promotes the proliferation of colorectal cancer cells by promoting cell cycle progression and inhibiting apoptosis.The results of the scratch test showed that SLC04A1-AS1 can promote the migration of colorectal cancer cells.Finally,tumor formation in nude mice confirmed that SLCO4A1-AS1 cound promote colorectal cancer proliferation in vivo.Conclusions:Our results demonstrate that SLCO4A1-AS1 has an indicative tumor suppressor role and appears to be a potential prognostic factor in colorectal cancer. |
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