Hepatoprotective Activities And Medianism Of Liviston Chinensis On Liver Injury

Objective:This study aims to explore the hepatoprotective effect and Possible mechanisms of TFFL in vivo and in vitro.Methods:a series of separation and purification techniques such as ethanolextraction,macroporous resin adsorption,and polyamide column separation and so on to be used to get the total flavonoids of Fructus livistonae chinensis.LO2 cells were treated with different concentrations of TFFL to evaluate the LO2 cell increment rate by CCK8 assay.The potential ability of different concentrations of TFFL pre-treatment effectively protecting LO2 cells from APAP-induced cell injury was evaluated by CCK8 assay.The levels of GSH、SOD、MDA、INOS、AST and ALT content in cell cytoplasm were examined.the protein expression of Bcl2 and Bax in mice liver was tested by Western Blot assay.6 week old male BABL/C mice were orally Pre-treatment with TFFL for consecutive ten days and acute liver injury was induced by APAP with a One shotintraperitoneal injection on the 10th day.The serum levels of ALT,AST and the levels of GSH,SOD,INOS and MDA in liver were measured.Mice liverpathological changes and morphological changes were observed.The inflammatory factors such as IL-1β、TNF-α、IL-6 were measured.the protein expression of NT、INOS、Nrf2、HO-1、Bax and Bcl2 in mice liver was tested by Western Blot assay.Results:Effect of cell Proliferation of TFFL on LO2cells in experiment showed that TFFL can Promote the Proliferation of LO2 in a given concentration in vivo.The study showed that TFFL protect LO2 against cell injury induced byacetaminophen by CCK8 assay;it attenuating the levels of ALT、AST、MDA and INOS,increasing the content of SOD、GSH.In the Western Blot assay,compared with the normal control group,the expression of Bax protein was significantly increased and the expression of Bcl2 protein was decreased in the modelgroup;eompared with the model group,the expression of Bax protein in each administration group decreased,and the dose was decreased.Dependence,while Bcl2Protein expression was elevated in the administration group.The vitro study Proved that TFFL protect mice against APAP-induced liver injury by markedly decreasing the serum levels of ALT and AST,attenuating thehistological damage of mice liver,elevating the levels of GSH and SOD,but decreasing the contents of MDA and INOS in liver,and the inflammatory factors such as IL-1β、TNF-α、IL-6 were decreased.In the Western Blot assay,the expression levels of INOS,NT,and Bax in the TFFL-administered group of mice were dose-dependently decreased,and the expression levels of Nrf2,HO-1,and Bcl2 in each TFFL group were increased..Conclusion:TFFL has a protective effect on liver injury induced by APAP,and its mechanism may be related with oxidative stress and nitrative stress.