The Role Of Melatonin In The Metastasis Of Papillary Thyroid Carcinoma

Objective:Serum melatonin levels were measured in patients with papillary thyroid carcinoma,multinodular goiter and healthy adults,and the expression levels of melatonin receptors MT1 and MT2 in thyroid cancer and adjacent normal thyroid tissues were measured..To evaluate the role of melatonin and the role of melatonin in the metastasis of papillary thyroid carcinoma in patients with papillary thyroid carcinoma.Method:1.Using ELISA,we measured serum melatonin levels in 81 patients with papillary thyroid carcinoma(PTC)and 20 patients with multinodular goiter(MNG)and 20 healthy adults,and Analysis of the relationship between melatonin and clinicopathological features of PTC.2.the expression of MT1 and MT2 in two subtypes of melatonin receptor in 81 cases of papillary thyroid carcinoma and adjacent tissues were detected by immunohistochemical SP method,and analyzed its the mean optical density(MOD)image by Image Pro Plusversion(IPP)image processing software.Result::1.ELISA test results:Serum melatonin concentration in patients with PTC was significantly higher than that in MNG patients and normal controls(P<0.05).The level of melatonin in the primary tumor T≥2cm group was significantly higher than that in the T<2cm group.Patients with positive cervical lymph nodes(N≥1)had significantly higher melatonin levels than lymph node negatives(N=0)(P<0.05).2.Immunohistochemistry results:The MT1 and MT2 receptors were expressed in both PTC and paracancerous tissues,mainly in the cell membrane and cytoplasm.The expression of MT1 receptor was low in the two groups,and there was no statistical difference.The expression of MT2 receptor in PTC tissues Significantly higher than the adjacent tissues(P<0.05),further studies showed that the expression of MT2 receptor in PTC tissues was associated with cervical lymph node metastasis,and the expression of MT2 receptor in PTC tissues with cervical lymph node metastasis was significantly lower than that without metastasis.(P < 0.05).Conclusion:1.Serum melatonin levels in PTC patients were higher than those in MNG and control groups,which may be associated with low malignancy of PTC.2.melatonin inhibits PTC metastasis,which exerts anti-PTC metastasis mainly through MT2 receptors.