Effects Of Different Doses Banxia Xiexin Decoction On PI3K/AKT Pathway In CAG Rats

Object To explore the effect mechanism of Banxia Xiexin Decoction on the PI3K/AKT pathway in a rat model of chronic atrophic gastritis,and to compare the effect difference between different doses.Methods SPF grade Wistar rats were randomly divided into a blank control group and a model group.The model group was used to establish a rat model of chronic atrophic gastritis.After the model was established,it was randomly divided into a model group,a Banxia Xiexin Decoction high-dose group,a Banxia Xiexin Decoction low-dose group,and a Vitacoenzyme group.Vitacoenzyme and different doses of Banxia Xiexin Decoction were used to intervene the rat model of chronic atrophic gastritis.The pathological changes of gastric mucosa in rats were observed.The expression of PTEN,VEGF,PIK3 CA,PIK3CB,p-AKT,AKT1/2/3,mTOR,BCL-2,GSK-3beta and FOXO3 A in gastric mucosa of rats were detected by immunohistochemical method.To explore the effect mechanism of Banxia Xiexin Decoction on PI3K/AKT in intervening chronic atrophic gastritis.Results Pathological sections showed that the arrangement of gastric glands in Banxia Xiexin Decoction intervention group was closer than that in model group,and the number of inflammatory cells in submucosa was significantly less.Immunohistochemical analysis showed that in the PI3K/AKT pathway,the expression of PIK3 CA was significantly decreased in the high dose group of Banxia Xiexin Decoction group(P<0.05),and the expression of PIK3 CB,AKT1/2/3 and p-AKT was significantly decreased.(P<0.01),the expression of AKT1/2/3 in the low-dose group of Banxia Xiexin Decoction was significantly lower than that in the model group(P<0.01).In terms of tumor suppressor,the expression levels of PTEN and FOXO3 A in the high-dose group of Banxia Xiexin Decoction group were significantly increased compared with the model group(P<0.01),while the expression of FOXO3 A in the low-dose group of Banxia Xiexin Decoction group was increased compared with the model group(P<0.05),and the expression of PTEN was significantly increased(P<0.01).In terms of cell growth regulation,the expression of mTOR was lower in the high-dose group of Banxia Xiexin Decoction group(P<0.05),the expression of BCL-2 was significantly decreased(P<0.01),and the GSK-3β was significantly increased(P<0.01).At the same time,the low dose of Banxia Xiexin Decoction decreased the expression of mTOR compared with the model group(P<0.05).In the regulation of vascular endothelial growth factor,the expression of VEGF in the high-dose group of Banxia Xiexin Decoction group was significantly lower than that in the model group(P<0.05).Conclusion(1)Banxia Xiexin Decoction can alleviate the inflammatory infiltration of the gastric submucosa and effectively improve the reduction and atrophy of the gastric gland in the chronic atrophic gastritis model.(2)Banxia Xiexin Decoction can inhibit the proliferation and accelerate apoptosis of gastric epithelial cells and lamina propria cells by inhibiting PI3K/AKT pathway,thereby preventing the mucosal epithelial cells and lamina propria cells from mutating.(3)High-dose Banxia Xiexin Decoction is superior to low-dose Banxia Xiexin Decoction in inhibiting PI3K/AKT pathway and improving pathological changes.