Bergenin Lowers The Uric Acid Level By Monitoring The Expression Of ABCG2 And GLUT9 In Kidney And Intestinal Tract

Objective:To determine if bergenin can lower urate by monitoring the expression of GLUT9 and ABCG2 in both kidney and intestine and prevent kidney injury.Method:Hyperuricemia was induced by oxonate and yeast extract in C57/BL6 mice.Mice were randomly divided into control group,hyperuricemia group,low dose bergenin group(40mg/kg),high dose bergenin group(80mg/kg),and allopurinol group.The serum uric acid level and serum creatine level was measured on 1st,7th,14th,21st day,and bowel fluid and urine wascollectedon the 21st day to calculate the urine fraction excretion of uric acid and intestinalexcretion of uric acid.Kidney and bowel was obtained on 21st day.mRNA expression examination was performed for ABCG2 and SLC2A9 from kidney and intestine.Western blot analysis was conducted to quantify the ABCG2 and GLUT9 expression.HE and MASSON stain were done for glomerulosclerosis,tubular injury and fibrosis.HE stain were done for intestine.Results:Uric acid levels in high dose bergenin treated mice decreased after 21 days and followed by significant increase of intestinal and urine uric acid excretion.Low dose bergenin could increase intestinal uric acid excretion.Higher expression of ABCG2 protein in both kidney and intestinal tractwas detected in the higher dose bergenin treated group compared with model group,while less expression of SLC2A9 was found in kidney and jejunum,but higher in ileum and colon.Higher dose bergenin group showed less fibrosis,tubular injury and glomerulosclerosis compared with hyperuricemia group.No inflammation cell is observed in intestine,the epithelial and interstitial tissue are normal.Conclusion:Bergenin can increase uric acid excretion of both intestine and kidney by monitoring the expression of ABCG2 and GLUT9.Bergenin can reduce renal injury.