Expression Of Cornulin In Cervical Cancer And Its Biological Function

Objective:This study aimed to demonstrate the expression of Cornulin(CRNN)in cervical cancer(CC)and its biological function in proliferation,migration and invasion.Methods:Cervical cancer tissues(21 cases)and normal cervical tissues(32 cases)were collected respectively from cervical cancer patients and uterine fibroids patients in the second Affiliated Hospital of Suzhou University from October 2016 to May 2018.The expression of CRNN in CC and normal cervical tissues were detected by Real-time quantitative PCR(qRT-PCR)and immunohistochemistry.The expression of CRNN in three CC cell lines(CaSki,HeLa and SiHa)and the normal cervical epithelial cell line(HcerEpic)were detected by qRT-PCR and Western blot.The stable HeLa cell lines with overexpression of CRNN and the stable Stable HcerEpic cell lines with knockdown of CRNN was established by the lentivirus-mediated method.The proliferation activity was tested by CCK-8 assays.Cell migration was detected using cell scratch assay.Cell invasion was detected using Matrigel-coated transwells.Results:CRNN is lowly expressed in cervical cancer tissues and highly expressed in normal cervical tissues;CRNN is lowly expressed in the three cervical cancer cell lines(CaSki,HeLa,and SiHa),but highly expressed in normal cervical epithelial cells(HcerEpic);In the cervical cancer cell lines,the overexpression of CRNN in Hela cells significantly reduced cell proliferation,migration and invasion,whereas cell proliferation,migration and invasion were markedly enhanced after the knockdown of CRNN in normal cervical epithelial cell line HcerEpic cells.Conclusion:CRNN is lowly expressed in cervical cancer tissues and cell lines.CRNN could affect the development of cervical cancer by mediating cell proliferation,migration and invasion.These data suggest that CRNN can be used as a candidate molecular marker and treatment target for the screening and prevention of cervical cancer.