| Objective:The effects of intra-articular injection of sodium hyaluronate on articular cartilage and subchondral bone in osteoarthritis of ovariectomized rats were investigated by establishing an in vivo model of OA combined with osteoporosis.Methods:Thirty female SD rats aged 3 months were randomly divided into 3 groups（10 rats in each group）:Sham-operated group,Bilateral anterior cruciate ligament transection in ovariectomised rats（OVX-ACLT group）,Bilateral anterior cruciate ligament transection in ovariectomised rats with intraarticular injection of sodium hyaluronate（the treatment group）.The sham-operation group without any treatment,but in OVX-ACLT group and treatment group with ovariectomized and transected anterior cruciate ligament of knee joint.Two weeks after OVX-ACLT,rats in the treatment group received intraarticular injection of sodium hyaluronate 50μg（0.05cc）once a week for 12 weeks.Rats in the OVX-ACLT group were injected with normal saline.The rats were sacrificed after 12 weeks of treatment.The CTX-I,TRACP5b,BALP levels were detected in blood,and Mankin scores of articular cartilage and Micro-CT bone mass analysis of subchondral bone were also evaluated.Results:（1）The content of CTX-I,TRACP5b,BALP in blood of OVX-ACLT group（43.68±22.25ng/ml,522.84±71.51pg/L,2.17±0.29μg/L）was significantly higher than that of sham operation group（18.21±8.06ng/ml,427.61±33.55pg/L,1.83±0.30μg/L）,The difference is statistically significant（P<0.01,P<0.05,P<0.05）,It indicates that osteoporosis exists in OVX-ACLT group,while that of treatment group（36.53±10.16ng/ml,484.00±51.37pg/L,1.92±0.26μg/L）was significantly lower than that of OVX-ACLT group（43.68±22.25ng/ml,522.84±71.51pg/L,2.17±0.29μg/L）,The difference is statistically significant（P<0.05,P<0.05,P<0.05）,This indicates that the treatment group inhibited the loss of subchondral bone mass.（2）Comparison of Mankin scores among three groups:the scores of the OVX-ACLT group（8.89±1.67）was significantly higher than that of the sham operation group（0.96±0.71）,The difference is st`atistically significant（P<0.01）,It indicates that the cartilage damage in OVX-ACLT group is serious,and the scores of the treatment group（2.56±1.42）was significantly lower than that of the OVX-ACLT group（8.89±1.67）,The difference is statistically significant（P<0.01）,This indicates that the treatment group delayed the progressive damage of cartilage.（3）The bone volume fraction（25.2451±2.6879%）and trabecular bone numbers(3.3394±0.4663mm-1)in OVX-ACLT group were significantly lower than those in sham-operated group(45.1209±3.2243%,5.5016±0.4170m m-1),The difference is statistically significant（P<0.01,P<0.01）,but the degree of bone trabecular separation（208.6545±24.6400μm）was significantly higher than that in sham-operated group（100.0690±7.0491μm）,The difference is statistically significant（P<0.01）,the trabecular thickness was not statistically different between the two groups（P>0.05）,This indicates the presence of osteoporosis in the subchondral bone of the OVX-ACLT group.The bone volume fraction（43.1300±4.3086%）and trabecular bone number(4.7820±0.4009mm-1)in treatment group were significantly higher than those in OVX-ACLT group(25.2451±2.6879%,3.3394±0.4663mm-1),The difference is statistically significant（P<0.01,P<0.01）,but the degree of bone trabecular separation（121.4571±19.6099μm）was significantly lower than that in OVX-ACLT group（208.6545±24.6400μm）,The difference is statistically significant（P<0.01）,the trabecular thickness was not statistically difference between the two groups（P>0.05）,This indicates that the strength of the subchondral bone in the treatment group is improved.Conclusion:Injection of sodium hyaluronate into articular cavity can improve the strength of subchondral bone and delay the degeneration of articular cartilage in ovariectomized rats. |
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