Preparation Of Gold Nanoparticles And Composites As Well As The Preliminary Study On Its Toxicity To Human Cervical Cancer SiHa Cells

Objective:In this study,gold nanoparticles(GNPs)are prepared,and cervical cancer specific binding peptide(CSP)is modified on the surface of gold nanoparticles(GNPs)to synthesize gold nanoparticles complex CSP-GNPs targeting human cervical cancer.The effects of different concentrations of GNPs and CSP-GNPs on the survival rate of human cervical cancer SiHa cells are studied,and their toxicity on SiHa cells are preliminarily discussed.The studies provide a reference for further research on targeted radiosensitization of cervical cancer.Methods:(1)GNPs are prepared by trisodium citrate reduction method,and CSPGNPs are synthesized by coupling CSP with GNPs with cysteine at the end via Au-S bond at room temperature.The effects of pH value of reaction system,volume ratio of polypeptide solution to gold nanoparticle solution and stirring speed on the dispersion and stability of CSP-GNPs are studied.The gold nanomaterials are tested and characterized by Malvern particle size analyzer,transmission electron microscopy(TEM),scanning electron microscopy(SEM),ultraviolet-visible spectrophotometer(UV-Vis),and inductively coupled plasma.The concentration of gold Au in the solution of gold nanoparticles is measured by ICP-AES.The connection between GNPs and CSP is verified by Fourier transform infrared spectroscopy(FTIR)and X-ray photoelectron spectroscopy(XPS).(2)Human cervical cancer SiHa cells in logarithmic growth phase are cultured in serum-free medium containing GNPs and CSP-GNPs of different concentrations for 24 and 48 hours.The toxicity of GNPs and CSP-GNPs to SiHa cell is detected by trypan blue staining.Results:(1)GNPs with an average particle size of 16.7 nm are successfully prepared.The concentration of Au in GNPs solution is 66.3 mg/L detected by ICP-AES.(2)The CSP-GNPs complex with good dispersion and stability is prepared.(3)Different concentrations of GNPs and CSP-GNPs do not significantly inhibit the proliferation of human cervical cancer SiHa cells(P > 0.05).Conclusion:(1)Gold nanoparticles prepared by trisodium citrate reduction method have good stability and dispersibility,and the particle size is easy to contol.(2)Successful synthesis of CSP-GNPs complexes with good stability and dispersion.(3)GNPs and CSPGNPs with concentrations less than 0.336mmol/L have no significant toxicity to human cervical cancer SiHa cells.