Artemisinin CAN Improve The Cognitive Dysfunction In Diabetic Mice Via Activating The PI3K/AKT Pathway

[Objective]To study the protective effect of artemisinin on the cognitive function of type 2 diabetic mice,and to explore the effect of artemisinin on the PI3K/Akt signaling pathway and the expression of hippocampal synapse related proteins.[Methods] 40 C57BL/6J mice aged 8 weeks were selected and bred adaptively for 1 week.After a week adaption,all the mice were randomly divided into the following 3 groups: normal control group,Model group and Artemisinin treatment group(40mg/kg);There were 10 mice in the normal control group,15 mice in the Model group and 15 mice in the Artemisinin treatment group.The normal control group were fed with normal diet,and the rest groups were fed with high-fat diet for 4 weeks.and then these mice were treated with streptozotocin(ip,100mg/kg)and fed with high-fat diet for 2 weeks.To confirm the establishment of the model of type 2 diabetic mice,the body weight and fasting blood glucose measurement,and abnormal glucose tolerance testof mice were performed.The Y maze novel object recognition experiment and the Morris water maze spatial exploration experiment were used to test the spatial learning and memory ability of mice.Mice were sacrificed and hippocampal specimens were immediately isolated and retained.Then,Western blot was used to detect the expression levels of PI3 K,total Akt,Akt phosphorylated protein and hippocampal synaptic related protein post-synaptic membrane dense protein(PSD-95)in mouse hippocampal tissue.The statistical data measured in the experiment were statistically processed.The software Sigmstat32 was used for statistical processing.[Results]1.Mice treated with high-fat diet combined with streptozotocin showed significant abnormalities in weight loss,hyperglycemia and glucose tolerance.2.The correct alternation rate of Y maze and the recognition index of novel object recognition in type 2 diabetic mice decreased obviously.The incubation period was significantly prolonged in Morris water maze.3.Artemisinin group could increase the correct alternation rate of Y maze in mice induced by high-fat diet combined with streptozotocin and reduce the recognition index of novel object recognition experiment and the incubation period of mice in water maze.4.In diabetic mice treated with artemisinin,the PI3 K AKT level,AKT phosphorylation level and the post-synaptic membrane dense protein(PSD-95)level were significantly increased in the hippocampus.Artemisinin significantly upregulates the PI3K/AKT pathway in the hippocampus of diabetic mice(P<0.05)and elevated the expression of hippocampal synapse related proteins(P<0.05).[Conclusion] Artemisinin can improve the cognitive dysfunction of type 2 diabetic mice,which may be related to the PI3K/Akt signaling pathway.