Circ₀001313 Inhibits Radiation Sensitivity Of Colon Cancer Cells By Targeting Negatively Regulate MiR-338-3p

[Objective]To explore the effects and mechanisms of Circ₀001313 on the radiation sensitivity of human colon cancer cells,and provide new targets and strategies for clinical radiotherapy of colon cancer.[Methods]1.Colon cancer cells SW480 and SW620 were irradiated at different doses,and the expression levels of Circ₀001313 and its downstream target genes in colon cancer cells were detected at different time points,which is to evaluate the effect of irradiation on the expression of Circ₀001313.2.The Circ₀001313 gene of colon cancer cells was silenced by RNA interference and to detect the expression of Circ₀001313 and miR-338-3p in colon cancer cells by RT-PCR.To investigate the effects of irradiation on cell viability,caspase-3 expression and colony colony in colon cancer cells when the Circ₀001313 gene was silenced,and to evaluate the relationship between Circ₀001313 and radiation sensitivity.3.Bioinformatics technology predicts downstream target genes of Circ₀001313.4.Interaction of Circ₀001313 with the downstream target gene miR-338-3p by the dual-luciferase reporter assay.5.RNA immunoprecipitation（RIP）experiments were performed to investigate whether Circ₀001313 and miR-338-3p could interact.6.The expression level of miR-338-3p was detected when Circ₀001313 was expressed differently.7.Using miR-338-3p inhibitor,and silencing the Circ₀001313 gene of colon cancer cells by RNA interference technology,to detect the expression of miR-338-3p by RT-PCR.To investigate the effects of miR-338-3p inhibitor on cell viability,caspase-3 expression and colony colony in colon cancer cells when the Circ₀001313 gene was silenced,and to evaluate the relationship between miR-338-3p inhibitor and radiation sensitivity.[Results]1.The expression of Circ₀001313 in colon cancer cells SW480 and SW620 under irradiation was increased in a dose and time-dependent manner;while the expression of miR-338-3p was decreased,which was opposite to Circ₀001313.2.Silence Circ₀001313 enhances the radio-sen-sitivity of colon cancer cells.3.miR-338-3p may be downstream target gene of Circ₀001313.4.Circ₀001313 can directly combined with miR-338-3p.5.Circ₀001313 can interact with miR-338-3p.6.Circ₀001313 negatively regulates the expression of miR-338-3p.7.miR-338-3p promoted the radiation sensitization of Silence Circ₀001313 to colon cancer cells.[Conclusions]1.Circ₀001313 inhibits the radiation sensitivity of colon cancer cells.2.Circ₀001313 inhibits radiation sensitivity of colon cancer cells by targeting Negatively Regulate miR-338-3p...