| Objective: Gestational diabetes mellitus(GDM)is a common disease during Pregnancy,which is first discovered or occurred during Pregnancy.In recent years,due to the westernization of dietary habits and the popularity of screening,the incidence of GDM in China is increasing year by year.However,the research on the pathogenesis of GDM is very limited.G Protein-coupled receptor 120(GPR120),as a long-chain unsaturated fatty acid activated receptor,has been reported that it regulates intracellular glucose metabolism and lipid metabolism,and that the dysfunction of GPR120 is associated with obesity,insulin resistance and impaired glucose tolerance,besides type 2 diabetes mellitus and fatty liver are closely related to the dysfunction of GPR120.However,there are few studies on the pathogenesis of GPR120 in GDM at present,so we mainly study the role of GPR120 in the pathogenesis of GDM.Methods:(1)Venous blood samples were collected from pregnant women with normal blood glucose(n = 33)and gestational diabetes mellitus(n = 29)who were enrolled in the Affiliated Hospital of Jiangnan University from July to December 2017.Venous blood samples were collected from 32 weeks of gestation,37 weeks of gestation and 2 days after delivery.The expression of GPR120 and FGF21 in white blood cells(WBCs)of two groups was detected by RT-PCR.(2)Lipid profiles in plasma of GDM and normal pregnancy were determined by LC-MS at 32 weeks of gestation,37 weeks of gestation and 2 days after delivery.The plasma sample sets were analyzed by PCA and OPLS-DA method.(3)In human leukocyte line THP-1 cells and leukocytes from human venous blood,the expression of GPR120 and FGF21 mRNA was detected after stimulated with different concentrations of TUG-891(agonist of GPR120).Results:(1)There was no significant difference in age,white blood cell count,C-reactive protein content,initial delivery ratio,and gender of delivery between the GDM group and the normal blood glucose group.The difference in BMI was P<0.05.There was no significant difference in the levels of HDL,TC,ALT,AST,ALP,CHE,CREA and UREA between GDM group and normal pregnancy group at 32 weeks of gestation.The levels of TG in GDM group was significantly higher in GDM group than that in the control group(P <0.05),and the level of LDL was significantly lower in GDM group than that in the control group(P < 0.05).There was no significant difference in the levels of HDL,ALT,AST,ALP,UREA and CREA between GDM group and normal pregnancy group at 37 weeks of gestation.The levels of TG and CHE in GDM group was significantly higher in GDM group than that in control group(P < 0.05),and the levels of LDL,TC was significantly lower in GDM group than that in control group(P < 0.05).There was no significant difference in the levels of HDL,LDL,TG,ALT,ALP,UREA and CREA between GDM group and normal pregnancy group attwo days after delivery.The levels of TC,AST in GDM group were significantly higher in GDM group than that in normal control group(P < 0.05),but the CHE level of pregnant women in GDM was significantly lower than that in normal control(P < 0.05).(2)At the 32 th week of gestation,5748 species of differential lipids were detected,of which 3287 species were up-regulated and 2461 species were down-regulated in GDM group.At the 37 th week of gestation,1126 species of differential lipids were detected,of which 383 species were up-regulated and 743 species were down-regulated in GDM group.At two days after delivery,1371 species of differential lipids were detected,of which 1323 species were up-regulated and 48 species were down-regulated in GDM group.During the three stages of pregnancy,8 species were significantly increased and 1 specie was significantly decreased in GDM patients.(3)The expression of serum GPR120 mRNA in GDM group was increased at the 32 th and 37 th week of gestation(P < 0.001).There was no significant difference in the expression of GPR120 mRNA between GDM group and normal pregnancy group at 2 days after delivery.At the same time,we analyzed the expression of GPR120 mRNA in the same group of patients at different stages of pregnancy.It was found that the expression of GPR120 mRNA was gradually increased in the GDM group during pregnancy,but decreased after delivery.In the normal pregnancy group,the expression of GPR120 mRNA was also increased during pregnancy,but it was also increased 2 days after delivery.(4)In this study,the expression and trend of FGF21 mRNA in leukocytes were consistent with GPR120 in the GDM group and the normal blood glucose group.(5)After stimulation with different concentrations of TUG891,the expression of GPR120 mRNA in THP-1 cells was increased obviously and the difference was statistically significant.With the increased of TUG891,the expression level of GPR120 mRNA increased gradually.The expression of GPR120 was the highest when the concentration of TUG891 was20μM,but there was no significant difference in the expression of GPR120 mRNA when the concentration of TUG891 was 10μM and 20μM.The change trend of FGF21 expression in THP-1 cells was the same as that of GPR120 mRNA expression.Conclusion:(1)During pregnancy,GDM patients have disordered lipid metabolism relative to pregnant women with normal blood glucose.(2)GPR120 and FGF21 mRNA expression levels were significantly increased in GDM patients at 32 weeks and 37 weeks of gestation.(3)There is a significant positive correlation between lipid metabolites and GPR120 and FGF21 mRNA expression levels.(4)Stimulation of GPR120 will induce the increase of FGF21 mRNA expression.It is suspected that the expression of GPR120 may be increased in gestational diabetes patients toinduce the increase of FGF21 mRNA expression,further control and regulation of GDM occurrence and development. |
PDF Full Text Request