Experimental Observation Of Programmed Necrosis In Ovarian Cancer Cells Induced By LPS And TNF-α

Objective To investigate the effects of LPS and TNF-α on programmed necrosis of human ovarian cancer cell line HEY,we verified the role of LPS and TNF-α in human ovarian cancer cell line HEY,and provided experimental basis for the treatment of ovarian cancer.Methods The human ovarian cancer cell line HEY was divided into three groups: a blank control group,a treatment group(LPS、TNF-α stimulation),and an antagonist group(LPS + TLR4、TNF-α antagonist).Real-time reverse transcription polymerase chain reaction(RT-PCR)was used to detect the expression of RIPK1,RIPK3 and MLKL mRNA.The levels of RIPK1,RIPK3,MLKL and p-MLKL protein in the experimental cells were detected by Western blot.Results The results of RT-PCR and Western blot showed that the expression levels of RIPK1 and RIPK3 mRNA and protein were higher in the LPS and TNF-α treatment groups than in the blank control group(P<0.05),and the difference was statistically significant.The expression of MLKL mRNA and protein was not statistically different between the two experimental groups.In a Western blot experiment,The expression of pMLKL protein was higher in the LPS,TNF-α treatment group and LPS+TLR4 and TNF-α antagonist groups than in the blank control group(P<0.05),The difference was statistically significant.Conclusion Both inflammatory factors LPS and TNF-α can promote programmed necrosis of human ovarian cancer cells.which verifies the role of LPS and TNF-α in ovarian cancer cells and provide new ideas and directions for clinical ovarian cancer treatment strategies.