Curcumin Combined With Vera Pammy Reverses Multidrug Resistance In Human Cholangiocarcinoma

Objective: The aim of this study was to investigate the reversal effect of curcumin and Vera Pammy on human cholangiocarcinoma multidrug-resistant cell line QBC939/ADM and its possible mechanism.Methods: The multidrug resistance cell model QBC939/ADM of human bile duct carcinoma was induced by concentration increasing method.The inhibitory rate of adriamycin and mitomycin on cell QBC939 and drug resistant cell QBC939/ADM in different concentrations was detected by CCK-8,and the IC50 value and resistance index were obtained to prove the establishment of the drug resistance model.With adriamycin as a control group,the experimental group was combined with curcumin,Vera Pammy and curcumin combined with adriamycin.The drug resistance of each group was detected by CCK-8 method.The rate of apoptosis was detected by flow cytometry,and the expression of MDR gene was detected by RT-PCR.Results: CCK-8 assay was used to detect the inhibitory rate of adriamycin and mitomycin on cell QBC939 and drug-resistant cell QBC939/ADM,respectively.After calculating the resistance index,the resistance index of QBC939/ADM cells to adriamycin and mitomycin was 9.3 and 4.26,respectively,compared with QBC939;CCK-8 was used to detect adriamycin for the experimental group and the test group.The cell survival rate in the group of adriamycin alone was(11.97±1.08)%,and the cell survival rate of adriamycin combined with Vera Pammy,curcumin and curcumin+ Vera Pammy respectively was(9.35±0.37)%、(8.07±0.53)%、(6.58±0.69)%respectively.The experimental group was compared with the control group,P < 0.05,the difference was compared with the control group.The apoptotic rate of adriamycin in the two groups was detected by flow cytometry.The apoptosis rate of adriamycin group alone was(6.45±0.78)%,and the apoptosis rate of adriamycin combined with Vera Pammy,curcumin and curcumin + Vera Pammy was(10.78±0.73)%、(14.68±0.42)%、(26.69±2.22)%,the experimental group compared with the control group,P < 0.05,the difference was statistically significant.The expression of MDR by RT-PCR was compared with that of adriamycin group(1).The MDRmRNA value of curcumin,verapamil and curcumin + verapamil group was(0.92±0.06),(0.88±0.06)and(0.66±0.10),and the expression was reduced.Conclusion: The reversal effect of curcumin and Vera Pammy on multidrug resistance of human bile duct carcinoma can increase the toxicity of doxorubicin to drug resistant cells.The apoptosis rate of drug-resistant cells in human bile duct cancer is significantly increased,the expression of MDR is reduced,and the combination of curcumin and Vera Pammy has a combined synergistic effect.However,the specific mechanism is still unclear and needs further research and exploration.