| The xylooligosaccharide(XOS)and ferulic acid(FA)have good effects on modifying gutmicrobiota and improving gut redox balance.In the present study,the mass ratio of XOS and FA of 5:1 and 10:1 were selected and their effects on modifying gut microbiota and improving gut redox in vitro and in vivo were studied.The results were obtained as follows:(1)Aging mouse faceal inoculum cultured with two different mass ratios of XOS and FAWhen the mass ratio of XOS and FA was 5:1,ROS(reactive oxygen species,ROS)generation rate(33.83±0.24)was significantly(p<0.05)lower than that of single XOS treatment(40.78±2.26)after 6 h fermentation.The pH values at 4 h(6.44±0.02)also showed great difference compared with that of single XOS treatment(6.77±0.01)Similarly,DPPH scavenging activity of 5:1 treatment(79.43±0.59%)was significantly(p<0.05)higher than cultured with XOS(72.68±0.84%)at 6 h.FA level in colon dropped rapidly from 50.40±0.27 mg/mL to 40.59±0.27 mg/mL after 5:1 treatment.When the mass ratio of XOS and FA was 10:1,ROS generation rate(126.1±1.0)was significantly(p<0.05)lower than that of single XOS treatment(150.55±2.13)at 8 h,and the pH value(p>0.05)was 6.31±0.01,6.26±0.01 at 6 h,respectively.A significant(p<0.05)difference was observed in DPPH scavenging activity between 10:1 treatment(95.22±0.98%)and the control(75.44±1.86%)at 8 h.FA level decreased gradually after 6h fermentation in 10:1 group dropped from 52.57±0.18 mg/mL to 51.73±0.16 mg/mL.In terms of butyric acid content,the same tendency was observed that 5:1 treatment showed higher content(38.0±0.2μg/mL)when comparing with 10:1 treatment(16.0±0.1μg/mL)after 6 h fermantation。The number of the Lactobacillus/E.coli ratios by 5:1 treatment(6.327)was significantly(p<0.05)higher than that of 10:1 treatment(1.957)at 8h.(2)Aging Sprague-Dawley(SD)rats induced by D-galactose followed by orally administered the standard feed add with 5:1 or 10:1 of XOS and FABoth body weight and DPPH scavenging activity of fresh fecal inoculum were selected to assese this aging rat model.The body weight(525.8±45.9 g)was insignificantly(p>0.05)higher than that of the control group(504.8±46.2g).However,DPPH scavenging activity(77.82±0.55%)was lower than the former(80.67±12.68%)After feeding aging rat with reproduced fodder,DPPH scavenging activity of the colonic content showed significant(p<0.05)difference between 5:1 treatment(85.29±1.00%)and 10:1 treatment(75.32±3.00%).ROS generation rate of liver treatment by 5:1(19.80±1.00)was lower(p<0.05)than that of 10:1 treatment(29.96±1.65).The number ofLactobacillus group divided by the number of E.coli group in colonic content were 7.46(5:1 group)and the number of Bifidobacterium group divided by the number of E.coli group were 0.0053(5:lgroup)respectively.They were both significantly(p<0.05)higher than that of 10:1 group(4.23)and(0.0033).Moreover,FA eontent in the serum(6.74±1.003μg/mL)administrated with 5:1 group were higher(p<0.05)than that of 10:1 group(6.02±1.201μg/mL).Butyric acid level in colonic content were 2.1±0.2μg/mL and 0.8±0.1μg/mL compared with 5:1 feed and 10:1 feed(p<0.05)Based on these observations,it can be concluded that the XOS and FA(5:1)treatment had better antioxidant effect on colonic oxidative stress than 10:1 treatment. |
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