The Relationship Between Islet β Cell Function And Metabolomics In Overweight T2DM Patients

Objective: A cross-sectional study was performed by metabolomics on patients with overweight type 2 diabetes at different stages of the disease.To investigate the correlation between islet β-cell dysfunction and metabolite changes in patients with overweight type 2 diabetes,with the aim of identifying potential metabolome biomarkers that can be used to assess islet beta cell function.Method: We selected adults with overweight(24≤BMI<28kg/m2)type 2 diabetes who were hospitalized in our hospital from May 2018 to November 2018(all meet the 1999 WHO diagnostic criteria for diabetes),and finally collected 60 cases.According to the duration of diabetes,the participants are divided into three groups,20 cases in each group,which were H1 group(≤ 5 years);H2 group(5 years < duration≤ 10 years);H3 group(duration > 10 years).Questionnaires,physical examinations,laboratory tests,and imaging studies were performed on all participants.The modified HOMA index was calculated with fasting C-peptide.Metabolite assays were performed by mass spectrometry.Statistical analysis was performed using SPSS 20.0 software.Results: 1.Homa-β and Visceral Fat Area(VFA)in patients with overweight type 2 diabetes are negatively correlated with duration.2.Correlation between HOMA index and metabolites in overweight type 2 diabetic patients(1)HOMA-β was negatively correlated with Ser and C14DC;positively correlated with Asp,Cys,Hcy,Pip,Pro and Val.(2)HOMA-IR is negatively correlated with C16-OH;positively correlated with C14.3.VFA in patients with overweight type 2 diabetes is positively correlated with Arg,Cys,Met,Pro,and C4 DC.Conclusions: 1.In patients with overweight T2 DM at different stages of disease,the visceral fat area gradually decreases with increasing disease duration.2.In patients with overweight T2 DM at different stages of disease,Ser,Asp,Cys,Hcy,Pip,Pro,Val,and C14 DC are associated with HOMA-β and are expected to be biomarkers for evaluating islet β-cell function.3.In patients with overweight T2 DM at different stages of disease,Arg,Cys,Met,Pro,and C4 DC are associated with in visceral fat area.