Experimental Study Of Pulmonary Artery Perfusion With Edaravone On Deep Hypothermic Circulatory Arrest

ObjectiveTo establish a deep hypothermic circulatory arrest model on Chinese miniature pigs,and to observe the protective effect of edaravone protecting solution on pulmonary artery perfusion in deep hypothermic circulatory arrest and to reseach their possible mechanism.Methods15 Chinese miniature pigs were randomly divided into 3 groups.In the control group,only the deep hypothermia cardiopulmonary model was established.Low potassium dextran(LPD)protective solution group,in addition to deep hypothermia cardiopulmonary bypass,perfused with low potassium dextran protection solution through the pulmonary artery.In the edaravone group,in addition to deep hypothermic cardiopulmonary bypass,a low potassium dextran protection solution containing edaravone was perfused through the pulmonary artery.Observed the contents of malondialdehyde(MDA)and superoxide dismutase(SOD)in venous blood at the baseline conditions,3 hours after DHCAcompleted,8 hours after DHCA completed,and 16 hours after DHCA stoped.The lung tissues of three groups of experimental animals were taken for HE staining,immunohistochemistry and gene detection,and the differences of each experimental group were observed.Results1.The concentration of malondialdehyde in the edaravone group was significantly lower than that in the LPD group and the control group,p<0.05 was statistically significant.2.The concentration of superoxide dismutase in the edaravone group was significantly higher than that in the LPD group and the control group,p<0.05 was statistically significant.3.The structure of HE-stained alveolar cells in the edaravone group was clear,and a small amount of inflammatory cells and red blood cells were observed.In the LPD group,the alveolar wall was slightly hyperplasia,and a small amount of inflammatory cells and red blood cells were observed.In the control group,the alveolar wall thickened significantly,and the tissue cells proliferated,showing inflammatory cells and red blood cells oozing out.4.The positive expression of ICAM-1 alveolar wall in immunohistochemical group of edaravone group was less than 30%and lightly stained.The positive expression of ICAM-1 alveolar wall in LPD group was 60%,which was lighter.The control composition was diffusely positive and the positive expression was 100%.5.The CT value of ICAM-1 gene detection in the edaravone group was significantly lower than that in the LPD group and the control group,p<0.05 was statistically significant.Conclusion1.Deep hypothermic circulatory arrest can cause lung damage2.This study established a model of deep hypothermia cardiopulmonary bypass in Chinese miniature pigs,and through the pulmonary artery perfusion of edaravone during transfusion,confirmed that edaravone has obvious protective effect on lung inj ury caused by deep hypothermic circulatory arrest.